Targeting MYB/MYBL1 gene rearrangements as diagnostic biomarkers in salivary cancers

Emily  Heathcote; Samuel Rack; Laura  Feeney; Karan Patel; Guy Betts; Clare Hodgson; Kate Garcez; Kevin J Harrington; Robert Metcalf

Targeting MYB/MYBL1 gene rearrangements as diagnostic biomarkers in salivary cancers

Emily Heathcote, Samuel Rack, Laura Feeney, Karan Patel, Guy Betts, Clare Hodgson, Kate Garcez, Kevin J Harrington, Robert Metcalf

Division of Immunology, Immunity to Infection and Respiratory Medicine

Research output: Contribution to conference › Abstract

Abstract

Lay abstract: In this study we validated MYB/MYBL1 detection using fluorescent in situ hybridization (FISH) probes for diagnosis of salivary adenoid cystic carcinoma (sACC). The study evaluated 62 patients with salivary cancer, where 49 had sACC, and 13 had non-ACC salivary cancer. FISH was used to detect MYB/MYBL1 rearrangement in patients with sACC, and DNA-based next-generation sequencing was used to confirm MYB gene rearrangement in a subgroup of 28 patients. Results showed that MYB/MYBL1 alterations were detected in 90% of sACC patients, making FISH probes a highly specific diagnostic biomarker for sACC. The study concludes that FISH probes have higher sensitivity than DNA-based NGS for detecting MYB gene fusions, making them useful for sACC diagnosis.

Original language	English
Publication status	Published - Mar 2023
Event	ECHNO (European Congress on Head and Neck Oncology) 23 - Lisbon Duration: 8 Mar 2023 → …

Conference

Conference	ECHNO (European Congress on Head and Neck Oncology) 23
City	Lisbon
Period	8/03/23 → …

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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@conference{9e48ac04681d41419f16c3d9b14542fc,

title = "Targeting MYB/MYBL1 gene rearrangements as diagnostic biomarkers in salivary cancers",

abstract = "Lay abstract: In this study we validated MYB/MYBL1 detection using fluorescent in situ hybridization (FISH) probes for diagnosis of salivary adenoid cystic carcinoma (sACC). The study evaluated 62 patients with salivary cancer, where 49 had sACC, and 13 had non-ACC salivary cancer. FISH was used to detect MYB/MYBL1 rearrangement in patients with sACC, and DNA-based next-generation sequencing was used to confirm MYB gene rearrangement in a subgroup of 28 patients. Results showed that MYB/MYBL1 alterations were detected in 90% of sACC patients, making FISH probes a highly specific diagnostic biomarker for sACC. The study concludes that FISH probes have higher sensitivity than DNA-based NGS for detecting MYB gene fusions, making them useful for sACC diagnosis.",

author = "Emily Heathcote and Samuel Rack and Laura Feeney and Karan Patel and Guy Betts and Clare Hodgson and Kate Garcez and Harrington, {Kevin J} and Robert Metcalf",

year = "2023",

month = mar,

language = "English",

note = "ECHNO (European Congress on Head and Neck Oncology) 23 ; Conference date: 08-03-2023",

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TY - CONF

T1 - Targeting MYB/MYBL1 gene rearrangements as diagnostic biomarkers in salivary cancers

AU - Heathcote, Emily

AU - Rack, Samuel

AU - Feeney, Laura

AU - Patel, Karan

AU - Betts, Guy

AU - Hodgson, Clare

AU - Garcez, Kate

AU - Harrington, Kevin J

AU - Metcalf, Robert

PY - 2023/3

Y1 - 2023/3

N2 - Lay abstract: In this study we validated MYB/MYBL1 detection using fluorescent in situ hybridization (FISH) probes for diagnosis of salivary adenoid cystic carcinoma (sACC). The study evaluated 62 patients with salivary cancer, where 49 had sACC, and 13 had non-ACC salivary cancer. FISH was used to detect MYB/MYBL1 rearrangement in patients with sACC, and DNA-based next-generation sequencing was used to confirm MYB gene rearrangement in a subgroup of 28 patients. Results showed that MYB/MYBL1 alterations were detected in 90% of sACC patients, making FISH probes a highly specific diagnostic biomarker for sACC. The study concludes that FISH probes have higher sensitivity than DNA-based NGS for detecting MYB gene fusions, making them useful for sACC diagnosis.

AB - Lay abstract: In this study we validated MYB/MYBL1 detection using fluorescent in situ hybridization (FISH) probes for diagnosis of salivary adenoid cystic carcinoma (sACC). The study evaluated 62 patients with salivary cancer, where 49 had sACC, and 13 had non-ACC salivary cancer. FISH was used to detect MYB/MYBL1 rearrangement in patients with sACC, and DNA-based next-generation sequencing was used to confirm MYB gene rearrangement in a subgroup of 28 patients. Results showed that MYB/MYBL1 alterations were detected in 90% of sACC patients, making FISH probes a highly specific diagnostic biomarker for sACC. The study concludes that FISH probes have higher sensitivity than DNA-based NGS for detecting MYB gene fusions, making them useful for sACC diagnosis.

M3 - Abstract

T2 - ECHNO (European Congress on Head and Neck Oncology) 23

Y2 - 8 March 2023

ER -

Targeting MYB/MYBL1 gene rearrangements as diagnostic biomarkers in salivary cancers

Abstract

Conference

UN SDGs

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