Taurine transport into fetal cord blood cells: Inhibition by cyclosporine A

Paul F. Speake; Christos S. Zipitis; Angela Houston; Stephen D&apos;Souza

doi:10.1016/j.jsgi.2004.05.004

Taurine transport into fetal cord blood cells: Inhibition by cyclosporine A

Paul F. Speake, Christos S. Zipitis, Angela Houston, Stephen D'Souza

Research output: Contribution to journal › Article › peer-review

Abstract

Pregnant women undergoing long-term organ transplant treatment have an increased incidence of delivering infants with intrauterine growth restriction (IUGR). Cyclosporine A is used as an immunosuppressant in such women and indirect evidence suggests that IUGR might result from an effect of cyclosporine A on amino acid transport by the placenta. In this study we tested the hypothesis that the transport of an essential amino acid, taurine, by fetal tissue other than the placenta is modulated by cyclosporine A. Cord blood cells (CBCs) were used to test this hypothesis as an easily obtainable fetal tissue. Transport of taurine into CBCs was measured using standard tracer flux assays. Uptake of [ 3H] taurine by CBCs was linear over 15 minutes (76.2 ± 16.6 fmol/10 6 cells/min, mean ± SEM, n = 6) and inhibitable by 10 mM β-alanine, a substrate of the system-β taurine transport protein (6.7 ± 1.0 fmol/10 6 cells/min, n = 6, P

Original language	English
Pages (from-to)	472-477
Number of pages	5
Journal	Journal of the Society for Gynecologic Investigation
Volume	11
Issue number	7
DOIs	https://doi.org/10.1016/j.jsgi.2004.05.004
Publication status	Published - Oct 2004

Keywords

amino acid transport
blood cells
cyclosporine A
Fetal
taurine

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10.1016/j.jsgi.2004.05.004

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title = "Taurine transport into fetal cord blood cells: Inhibition by cyclosporine A",

abstract = "Pregnant women undergoing long-term organ transplant treatment have an increased incidence of delivering infants with intrauterine growth restriction (IUGR). Cyclosporine A is used as an immunosuppressant in such women and indirect evidence suggests that IUGR might result from an effect of cyclosporine A on amino acid transport by the placenta. In this study we tested the hypothesis that the transport of an essential amino acid, taurine, by fetal tissue other than the placenta is modulated by cyclosporine A. Cord blood cells (CBCs) were used to test this hypothesis as an easily obtainable fetal tissue. Transport of taurine into CBCs was measured using standard tracer flux assays. Uptake of [ 3H] taurine by CBCs was linear over 15 minutes (76.2 ± 16.6 fmol/10 6 cells/min, mean ± SEM, n = 6) and inhibitable by 10 mM β-alanine, a substrate of the system-β taurine transport protein (6.7 ± 1.0 fmol/10 6 cells/min, n = 6, P",

keywords = "amino acid transport, blood cells, cyclosporine A, Fetal, taurine",

author = "Speake, {Paul F.} and Zipitis, {Christos S.} and Angela Houston and Stephen D'Souza",

year = "2004",

month = oct,

doi = "10.1016/j.jsgi.2004.05.004",

language = "English",

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TY - JOUR

T1 - Taurine transport into fetal cord blood cells: Inhibition by cyclosporine A

AU - Speake, Paul F.

AU - Zipitis, Christos S.

AU - Houston, Angela

AU - D'Souza, Stephen

PY - 2004/10

Y1 - 2004/10

N2 - Pregnant women undergoing long-term organ transplant treatment have an increased incidence of delivering infants with intrauterine growth restriction (IUGR). Cyclosporine A is used as an immunosuppressant in such women and indirect evidence suggests that IUGR might result from an effect of cyclosporine A on amino acid transport by the placenta. In this study we tested the hypothesis that the transport of an essential amino acid, taurine, by fetal tissue other than the placenta is modulated by cyclosporine A. Cord blood cells (CBCs) were used to test this hypothesis as an easily obtainable fetal tissue. Transport of taurine into CBCs was measured using standard tracer flux assays. Uptake of [ 3H] taurine by CBCs was linear over 15 minutes (76.2 ± 16.6 fmol/10 6 cells/min, mean ± SEM, n = 6) and inhibitable by 10 mM β-alanine, a substrate of the system-β taurine transport protein (6.7 ± 1.0 fmol/10 6 cells/min, n = 6, P

AB - Pregnant women undergoing long-term organ transplant treatment have an increased incidence of delivering infants with intrauterine growth restriction (IUGR). Cyclosporine A is used as an immunosuppressant in such women and indirect evidence suggests that IUGR might result from an effect of cyclosporine A on amino acid transport by the placenta. In this study we tested the hypothesis that the transport of an essential amino acid, taurine, by fetal tissue other than the placenta is modulated by cyclosporine A. Cord blood cells (CBCs) were used to test this hypothesis as an easily obtainable fetal tissue. Transport of taurine into CBCs was measured using standard tracer flux assays. Uptake of [ 3H] taurine by CBCs was linear over 15 minutes (76.2 ± 16.6 fmol/10 6 cells/min, mean ± SEM, n = 6) and inhibitable by 10 mM β-alanine, a substrate of the system-β taurine transport protein (6.7 ± 1.0 fmol/10 6 cells/min, n = 6, P

KW - amino acid transport

KW - blood cells

KW - cyclosporine A

KW - Fetal

KW - taurine

U2 - 10.1016/j.jsgi.2004.05.004

DO - 10.1016/j.jsgi.2004.05.004

M3 - Article

SN - 1071-5576

VL - 11

SP - 472

EP - 477

JO - Journal of the Society for Gynecologic Investigation

JF - Journal of the Society for Gynecologic Investigation

IS - 7

ER -

Taurine transport into fetal cord blood cells: Inhibition by cyclosporine A

Abstract

Keywords

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