TY - JOUR
T1 - TB PCR in the early diagnosis of tuberculous meningitis
T2 - Evaluation of the Roche semi-automated COBAS amplicor MTB test with reference to the manual Amplicor MTB PCR test
AU - Bonington, A.
AU - Strang, J. I.G.
AU - Klapper, P. E.
AU - Hood, S. V.
AU - Parish, A.
AU - Swift, P. J.
AU - Damba, J.
AU - Stevens, H.
AU - Sawyer, L.
AU - Potgieter, G.
AU - Bailey, A.
AU - Wilkins, E. G.L.
PY - 2000/8/10
Y1 - 2000/8/10
N2 - Setting: Cecilia Makiwane Hospital, Mdantsane, Eastern Cape, Republic of South Africa. Objective: To assess the role of the semi-automated Roche COBAS AMPLICOR(TM) Mycobacterium tuberculosis PCR test in the diagnosis of tuberculous meningitis (TBM). Design: Eighty-three specimens of cerebrospinal fluid (CSF) were collected prospectively from 69 patients with suspected TBM. The COBAS AMPLICOR TB PCR test was compared with the manual AMPLICOR(TM) TB PCR test, clinical and cerebrospinal fluid (CSF) findings, direct ZN smear and radiometric TB culture. Results: CSF from 7/40 (17.5%) patients treated for TBM were positive by TB COBAS AMPLICOR(TM). The sensitivity of the test was not significantly different (p = 0.375) from the manual TB AMPLICOR(TM) PCR test. The comparative sensitivities of the TB COBAS AMPLICOR(TM) PCR and the manual AMPLICOR PCR for detecting cases of definite and probable TBM from CSF collected within 9 days of commencing antituberculosis treatment were 40% and 60% respectively. All 29 patients not treated for TBM were negative by COBAS AMPLICOR(TM), giving a specificity of 100%. Conclusion: The COBAS AMPLICOR(TM) TB PCR test is a rapid and highly specific diagnostic test for TBM. However, there was a non-significant trend favouring slightly greater sensitivity using the manual AMPLICOR(TM) TB PCR test. (C) 2000 Harcourt Publishers Ltd.
AB - Setting: Cecilia Makiwane Hospital, Mdantsane, Eastern Cape, Republic of South Africa. Objective: To assess the role of the semi-automated Roche COBAS AMPLICOR(TM) Mycobacterium tuberculosis PCR test in the diagnosis of tuberculous meningitis (TBM). Design: Eighty-three specimens of cerebrospinal fluid (CSF) were collected prospectively from 69 patients with suspected TBM. The COBAS AMPLICOR TB PCR test was compared with the manual AMPLICOR(TM) TB PCR test, clinical and cerebrospinal fluid (CSF) findings, direct ZN smear and radiometric TB culture. Results: CSF from 7/40 (17.5%) patients treated for TBM were positive by TB COBAS AMPLICOR(TM). The sensitivity of the test was not significantly different (p = 0.375) from the manual TB AMPLICOR(TM) PCR test. The comparative sensitivities of the TB COBAS AMPLICOR(TM) PCR and the manual AMPLICOR PCR for detecting cases of definite and probable TBM from CSF collected within 9 days of commencing antituberculosis treatment were 40% and 60% respectively. All 29 patients not treated for TBM were negative by COBAS AMPLICOR(TM), giving a specificity of 100%. Conclusion: The COBAS AMPLICOR(TM) TB PCR test is a rapid and highly specific diagnostic test for TBM. However, there was a non-significant trend favouring slightly greater sensitivity using the manual AMPLICOR(TM) TB PCR test. (C) 2000 Harcourt Publishers Ltd.
UR - http://www.scopus.com/inward/record.url?scp=0033730190&partnerID=8YFLogxK
U2 - 10.1054/tuld.2000.0246
DO - 10.1054/tuld.2000.0246
M3 - Article
C2 - 11052908
AN - SCOPUS:0033730190
SN - 0962-8479
VL - 80
SP - 191
EP - 196
JO - Tubercle and Lung Disease
JF - Tubercle and Lung Disease
IS - 4-5
ER -
