Tcof1/Treacle is required for neural crest cell formation and proliferation deficiencies that cause craniofacial abnormalities

Jill Dixon, Natalie C. Jones, Lisa L. Sandell, Sachintha M. Jayasinghe, Jennifer Crane, Jean Philippe Rey, Michael J. Dixon, Paul A. Trainor

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Neural crest cells are a migratory cell population that give rise to the majority of the cartilage, bone, connective tissue, and sensory ganglia in the head. Abnormalities in the formation, proliferation, migration, and differentiation phases of the neural crest cell life cycle can lead to craniofacial malformations, which constitute one-third of all congenital birth defects. Treacher Collins syndrome (TCS) is characterized by hypoplasia of the facial bones, cleft palate, and middle and external ear defects. Although TCS results from autosomal dominant mutations of the gene TCOF1, the mechanistic origins of the abnormalities observed in this condition are unknown, and the function of Treacle, the protein encoded by TCOF1, remains poorly understood. To investigate the developmental basis of TCS we generated a mouse model through germ-line mutation of Tcof1. Haploinsufficiency of Tcof1 leads to a deficiency in migrating neural crest cells, which results in severe craniofacial malformations. We demonstrate that Tcof1/Treacle is required cell-autonomously for the formation and proliferation of neural crest cells. Tcof1/Treacle regulates proliferation by controlling the production of mature ribosomes. Therefore, Tcof1/Treacle is a unique spatiotemporal regulator of ribosome biogenesis, a deficiency that disrupts neural crest cell formation and proliferation, causing the hypoplasia characteristic of TCS craniofacial anomalies. © 2006 by The National Academy of Sciences of the USA.
    Original languageEnglish
    Pages (from-to)13403-13408
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume103
    Issue number36
    DOIs
    Publication statusPublished - 5 Sept 2006

    Keywords

    • Craniofacial development
    • Embryo
    • Mouse
    • Treacher Collins syndrome

    Fingerprint

    Dive into the research topics of 'Tcof1/Treacle is required for neural crest cell formation and proliferation deficiencies that cause craniofacial abnormalities'. Together they form a unique fingerprint.

    Cite this