Temperature acclimation has no effect on ryanodine receptor expression or subcellular localization in rainbow trout heart

Rikke Birkedal; Jennifer Christopher; Angela Thistlethwaite; Holly A. Shiels

doi:10.1007/s00360-009-0377-x

Temperature acclimation has no effect on ryanodine receptor expression or subcellular localization in rainbow trout heart

Rikke Birkedal, Jennifer Christopher, Angela Thistlethwaite, Holly A. Shiels

Research output: Contribution to journal › Article › peer-review

Abstract

In cardiomyocytes, ryanodine receptors (RYRs) mediate Ca2+-induced Ca2+-release (CICR) from the sarcoplasmic reticulum (SR) during excitation-contraction (e-c) coupling. In rainbow trout heart, the relative importance of CICR increases with cold-acclimation. Thus, the aim of this study was to investigate the effect of temperature acclimation (4, 11 and 18°C) on RYR intracellular localization and expression density. We used immunocytochemistry to assess intracellular localization in ventricular myocytes and Western blotting to assess RYR expression in both atrial and ventricular tissue. In ventricular myocytes, RYRs were localized peripherally in transverse bands aligning with sarcomeric m-lines and centrally around mitochondria and the nucleus. Localization did not change with temperature acclimation. RYR expression was also unaffected by temperature acclimation. The localization of RYRs at the m-line is similar to neonatal mammalian cardiomyocytes. We suggest this positioning is indicative of myocytes which rely predominantly on transsarcolemmal Ca2+-influx, rather than CICR, during e-c coupling. © Springer-Verlag 2009.

Original language	English
Pages (from-to)	961-969
Number of pages	8
Journal	Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume	179
Issue number	8
DOIs	https://doi.org/10.1007/s00360-009-0377-x
Publication status	Published - Oct 2009

Keywords

CICR
Excitation-contraction coupling
Immunocytochemistry
Oncorhynchus mykiss
Ryanodine receptor
Sarcoplasmic reticulum

Access to Document

10.1007/s00360-009-0377-x

Cite this

@article{e767201d545c4ed286981f0fc1600696,

title = "Temperature acclimation has no effect on ryanodine receptor expression or subcellular localization in rainbow trout heart",

abstract = "In cardiomyocytes, ryanodine receptors (RYRs) mediate Ca2+-induced Ca2+-release (CICR) from the sarcoplasmic reticulum (SR) during excitation-contraction (e-c) coupling. In rainbow trout heart, the relative importance of CICR increases with cold-acclimation. Thus, the aim of this study was to investigate the effect of temperature acclimation (4, 11 and 18°C) on RYR intracellular localization and expression density. We used immunocytochemistry to assess intracellular localization in ventricular myocytes and Western blotting to assess RYR expression in both atrial and ventricular tissue. In ventricular myocytes, RYRs were localized peripherally in transverse bands aligning with sarcomeric m-lines and centrally around mitochondria and the nucleus. Localization did not change with temperature acclimation. RYR expression was also unaffected by temperature acclimation. The localization of RYRs at the m-line is similar to neonatal mammalian cardiomyocytes. We suggest this positioning is indicative of myocytes which rely predominantly on transsarcolemmal Ca2+-influx, rather than CICR, during e-c coupling. {\textcopyright} Springer-Verlag 2009.",

keywords = "CICR, Excitation-contraction coupling, Immunocytochemistry, Oncorhynchus mykiss, Ryanodine receptor, Sarcoplasmic reticulum",

author = "Rikke Birkedal and Jennifer Christopher and Angela Thistlethwaite and Shiels, {Holly A.}",

year = "2009",

month = oct,

doi = "10.1007/s00360-009-0377-x",

language = "English",

volume = "179",

pages = "961--969",

journal = "Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology",

issn = "0174-1578",

publisher = "Springer Nature",

number = "8",

}

Temperature acclimation has no effect on ryanodine receptor expression or subcellular localization in rainbow trout heart. / Birkedal, Rikke; Christopher, Jennifer; Thistlethwaite, Angela et al.
In: Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology, Vol. 179, No. 8, 10.2009, p. 961-969.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Temperature acclimation has no effect on ryanodine receptor expression or subcellular localization in rainbow trout heart

AU - Birkedal, Rikke

AU - Christopher, Jennifer

AU - Thistlethwaite, Angela

AU - Shiels, Holly A.

PY - 2009/10

Y1 - 2009/10

N2 - In cardiomyocytes, ryanodine receptors (RYRs) mediate Ca2+-induced Ca2+-release (CICR) from the sarcoplasmic reticulum (SR) during excitation-contraction (e-c) coupling. In rainbow trout heart, the relative importance of CICR increases with cold-acclimation. Thus, the aim of this study was to investigate the effect of temperature acclimation (4, 11 and 18°C) on RYR intracellular localization and expression density. We used immunocytochemistry to assess intracellular localization in ventricular myocytes and Western blotting to assess RYR expression in both atrial and ventricular tissue. In ventricular myocytes, RYRs were localized peripherally in transverse bands aligning with sarcomeric m-lines and centrally around mitochondria and the nucleus. Localization did not change with temperature acclimation. RYR expression was also unaffected by temperature acclimation. The localization of RYRs at the m-line is similar to neonatal mammalian cardiomyocytes. We suggest this positioning is indicative of myocytes which rely predominantly on transsarcolemmal Ca2+-influx, rather than CICR, during e-c coupling. © Springer-Verlag 2009.

AB - In cardiomyocytes, ryanodine receptors (RYRs) mediate Ca2+-induced Ca2+-release (CICR) from the sarcoplasmic reticulum (SR) during excitation-contraction (e-c) coupling. In rainbow trout heart, the relative importance of CICR increases with cold-acclimation. Thus, the aim of this study was to investigate the effect of temperature acclimation (4, 11 and 18°C) on RYR intracellular localization and expression density. We used immunocytochemistry to assess intracellular localization in ventricular myocytes and Western blotting to assess RYR expression in both atrial and ventricular tissue. In ventricular myocytes, RYRs were localized peripherally in transverse bands aligning with sarcomeric m-lines and centrally around mitochondria and the nucleus. Localization did not change with temperature acclimation. RYR expression was also unaffected by temperature acclimation. The localization of RYRs at the m-line is similar to neonatal mammalian cardiomyocytes. We suggest this positioning is indicative of myocytes which rely predominantly on transsarcolemmal Ca2+-influx, rather than CICR, during e-c coupling. © Springer-Verlag 2009.

KW - CICR

KW - Excitation-contraction coupling

KW - Immunocytochemistry

KW - Oncorhynchus mykiss

KW - Ryanodine receptor

KW - Sarcoplasmic reticulum

U2 - 10.1007/s00360-009-0377-x

DO - 10.1007/s00360-009-0377-x

M3 - Article

C2 - 19544062

SN - 0174-1578

VL - 179

SP - 961

EP - 969

JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

IS - 8

ER -

Temperature acclimation has no effect on ryanodine receptor expression or subcellular localization in rainbow trout heart

Abstract

Keywords

Access to Document

Fingerprint

Cite this