Tenocyte contraction induces crimp formation in tendon-like tissue

Andreas Herchenhan, Nicholas S. Kalson, David F. Holmes, Patrick Hill, Karl E. Kadler, Lee Margetts

    Research output: Contribution to journalArticlepeer-review


    Tendons are composed of longitudinally aligned collagen fibrils arranged in bundles with an undulating pattern, called crimp. The crimp structure is established during embryonic development and plays a vital role in the mechanical behaviour of tendon, acting as a shock-absorber during loading. However, the mechanism of crimp formation is unknown, partly because of the difficulties of studying tendon development in vivo. Here, we used a 3D cell culture system in which embryonic tendon fibroblasts synthesise a tendonlike construct comprised of collagen fibrils arranged in parallel bundles. Investigations using polarised light microscopy, scanning electron microscopy and fluorescence microscopy showed that tendon constructs contained a regular pattern of wavy collagen fibrils. Tensile testing indicated that this superstructure was a form of embryonic crimp producing a characteristic toe region in the stress-strain curves. Furthermore, contraction of tendon fibroblasts was the critical factor in the buckling of collagen fibrils during the formation of the crimp structure. Using these biological data, a finite element model was built that mimics the contraction of the tendon fibroblasts and monitors the response of the Extracellular matrix. The results show that the contraction of the fibroblasts is a sufficient mechanical impulse to build a planar wavy pattern. Furthermore, the value of crimp wavelength was determined by the mechanical properties of the collagen fibrils and inter-fibrillarmatrix. Increasing fibril stiffness combined with constant matrix stiffness led to an increase in crimp wavelength. The data suggest a novel mechanism of crimp formation, and the finite element model indicates the minimum requirements to generate a crimp structure in embryonic tendon. © Springer-Verlag 2011.
    Original languageEnglish
    Pages (from-to)449-459
    Number of pages10
    JournalBiomechanics and modeling in mechanobiology
    Issue number3-4
    Publication statusPublished - Mar 2012


    • Chick embryonic tendon
    • Collagen
    • Crimp
    • Extracellular matrix
    • Finite element modeling
    • Tension


    Dive into the research topics of 'Tenocyte contraction induces crimp formation in tendon-like tissue'. Together they form a unique fingerprint.

    Cite this