Terminal excimer and exciplex fluorescence a sensitive oligonucleotide probe

Abdul Fatah Gbaj, Samia Hawisa, Lindsey Walsh, Candy Rogert, Alireza Sardarian, Elena V. Bichenkova, Laura L. Etchells, Kenneth T. Douglas

    Research output: Contribution to journalArticlepeer-review


    The development of a new sensitive, safe and easy to use exciplex probes system for the detection of DNA is of relevant importance. The aim of this study was to develop a fluorescence- based technique to work in homogenous, and then heterogeneous assays systems. Exciplex signal with DNA hybridisation were determined in solution using fluorescence measurements and complementary fluorophore-labeled oligonucleotides. One oligonucleotide probe, was labeled with a 5′-terminal pyrene, and the other was labelled with 3′-terminal naphthalene. The juxtaposition of the two labels in double-stranded complexes, results in a strong exciplex signal at a longer wavelength (∼ 480nm) thereby providing the means to differentiate single-stranded DNA from double-stranded DNA. Since measurements were based on fluorescence, DNA denaturation and association could be monitored routinely at low DNA concentrations. The importance of the presence of various counter cations (Na+, K+ and Mg2+) on exciplex signal intensity was evaluated. The results obtained represent the first case of an oligonucleotide terminally located probe system based on exciplex fluorescence emission.
    Original languageEnglish
    Pages (from-to)250-257
    Number of pages7
    JournalJamahiriya Medical Journal
    Issue number4
    Publication statusPublished - Dec 2009


    • DNA detection
    • Exciplex
    • Fluorescence
    • Stokes shift
    • Terminally located probe systems


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