Test system for quantification of stop codon suppression by selenocysteine insertion in mammalian cell lines

John Mccarthy; H. Kollmus; J. E G McCarthy; L. Flohe

Test system for quantification of stop codon suppression by selenocysteine insertion in mammalian cell lines

John Mccarthy, H. Kollmus, J. E G McCarthy, L. Flohe

Research output: Contribution to journal › Article › peer-review

Abstract

A convenient test system was designed to investigate the efficiencies of selenocysteine inserting sequences (SECIS) responsible for the cotranslational incorporation of selenocysteine into selenoproteins of mammals. It comprises an expression vector in which the lacZ and luc genes are separated by an in-frame TGA stop codon. The coding regions are followed by a multicloning region allowing exchange of putative SECIS elements. Stop codon suppression associated with selenocysteine incorporation is readily estimated on the basis of relative luciferase activity measurements, thus providing a measure of SECIS efficiency.

Original language	English
Pages (from-to)	114-117
Number of pages	3
Journal	Zeitschrift fuer Ernaehrungswissenschaft
Volume	37
Issue number	1
Publication status	Published - 1998

Keywords

SECIS element
Selenocysteine
Translation termination

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abstract = "A convenient test system was designed to investigate the efficiencies of selenocysteine inserting sequences (SECIS) responsible for the cotranslational incorporation of selenocysteine into selenoproteins of mammals. It comprises an expression vector in which the lacZ and luc genes are separated by an in-frame TGA stop codon. The coding regions are followed by a multicloning region allowing exchange of putative SECIS elements. Stop codon suppression associated with selenocysteine incorporation is readily estimated on the basis of relative luciferase activity measurements, thus providing a measure of SECIS efficiency.",

keywords = "SECIS element, Selenocysteine, Translation termination",

author = "John Mccarthy and H. Kollmus and McCarthy, {J. E G} and L. Flohe",

year = "1998",

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journal = "Zeitschrift fuer Ernaehrungswissenschaft",

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T1 - Test system for quantification of stop codon suppression by selenocysteine insertion in mammalian cell lines

AU - Mccarthy, John

AU - Kollmus, H.

AU - McCarthy, J. E G

AU - Flohe, L.

PY - 1998

Y1 - 1998

N2 - A convenient test system was designed to investigate the efficiencies of selenocysteine inserting sequences (SECIS) responsible for the cotranslational incorporation of selenocysteine into selenoproteins of mammals. It comprises an expression vector in which the lacZ and luc genes are separated by an in-frame TGA stop codon. The coding regions are followed by a multicloning region allowing exchange of putative SECIS elements. Stop codon suppression associated with selenocysteine incorporation is readily estimated on the basis of relative luciferase activity measurements, thus providing a measure of SECIS efficiency.

AB - A convenient test system was designed to investigate the efficiencies of selenocysteine inserting sequences (SECIS) responsible for the cotranslational incorporation of selenocysteine into selenoproteins of mammals. It comprises an expression vector in which the lacZ and luc genes are separated by an in-frame TGA stop codon. The coding regions are followed by a multicloning region allowing exchange of putative SECIS elements. Stop codon suppression associated with selenocysteine incorporation is readily estimated on the basis of relative luciferase activity measurements, thus providing a measure of SECIS efficiency.

KW - SECIS element

KW - Selenocysteine

KW - Translation termination

M3 - Article

C2 - 9558741

SN - 1435-1293

VL - 37

SP - 114

EP - 117

JO - Zeitschrift fuer Ernaehrungswissenschaft

JF - Zeitschrift fuer Ernaehrungswissenschaft

IS - 1

ER -

Test system for quantification of stop codon suppression by selenocysteine insertion in mammalian cell lines

Abstract

Keywords

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