The Arabidopsis peptide kiss of death is an inducer of programmed cell death

Robert Blanvillain; Bennett Young; Yao Min Cai; Valérie Hecht; Fabrice Varoquaux; Valérie Delorme; Jean Marc Lancelin; Michel Delseny; Patrick Gallois

doi:10.1038/emboj.2011.14

The Arabidopsis peptide kiss of death is an inducer of programmed cell death

Robert Blanvillain, Bennett Young, Yao Min Cai, Valérie Hecht, Fabrice Varoquaux, Valérie Delorme, Jean Marc Lancelin, Michel Delseny, Patrick Gallois

Research output: Contribution to journal › Article › peer-review

Abstract

Programmed cell death (PCD) has a key role in defence and development of all multicellular organisms. In plants, there is a large gap in our knowledge of the molecular machinery involved at the various stages of PCD, especially the early steps. Here, we identify kiss of death (KOD) encoding a 25-amino-acid peptide that activates a PCD pathway in Arabidopsis thaliana. Two mutant alleles of KOD exhibited a reduced PCD of the suspensor, a single file of cells that support embryo development, and a reduced PCD of root hairs after a 55 °C heat shock. KOD expression was found to be inducible by biotic and abiotic stresses. Furthermore, KOD expression was sufficient to cause death in leaves or seedlings and to activate caspase-like activities. In addition, KOD-induced PCD required light in leaves and was repressed by the PCD-suppressor genes AtBax inhibitor 1 and p35. KOD expression resulted in depolarization of the mitochondrial membrane, placing KOD above mitochondria dysfunction, an early step in plant PCD. A KOD-GFP fusion, however, localized in the cytosol of cells and not mitochondria. © 2011 European Molecular Biology Organization | All Rights Reserved.

Original language	English
Pages (from-to)	1173-1183
Number of pages	10
Journal	EMBO Journal
Volume	30
Issue number	6
DOIs	https://doi.org/10.1038/emboj.2011.14
Publication status	Published - 16 Mar 2011

Keywords

BAX inhibitor 1
caspase-like
embryogenesis
p35

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10.1038/emboj.2011.14

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title = "The Arabidopsis peptide kiss of death is an inducer of programmed cell death",

abstract = "Programmed cell death (PCD) has a key role in defence and development of all multicellular organisms. In plants, there is a large gap in our knowledge of the molecular machinery involved at the various stages of PCD, especially the early steps. Here, we identify kiss of death (KOD) encoding a 25-amino-acid peptide that activates a PCD pathway in Arabidopsis thaliana. Two mutant alleles of KOD exhibited a reduced PCD of the suspensor, a single file of cells that support embryo development, and a reduced PCD of root hairs after a 55 °C heat shock. KOD expression was found to be inducible by biotic and abiotic stresses. Furthermore, KOD expression was sufficient to cause death in leaves or seedlings and to activate caspase-like activities. In addition, KOD-induced PCD required light in leaves and was repressed by the PCD-suppressor genes AtBax inhibitor 1 and p35. KOD expression resulted in depolarization of the mitochondrial membrane, placing KOD above mitochondria dysfunction, an early step in plant PCD. A KOD-GFP fusion, however, localized in the cytosol of cells and not mitochondria. {\textcopyright} 2011 European Molecular Biology Organization | All Rights Reserved.",

keywords = "BAX inhibitor 1, caspase-like, embryogenesis, p35",

author = "Robert Blanvillain and Bennett Young and Cai, {Yao Min} and Val{\'e}rie Hecht and Fabrice Varoquaux and Val{\'e}rie Delorme and Lancelin, {Jean Marc} and Michel Delseny and Patrick Gallois",

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T1 - The Arabidopsis peptide kiss of death is an inducer of programmed cell death

AU - Blanvillain, Robert

AU - Young, Bennett

AU - Cai, Yao Min

AU - Hecht, Valérie

AU - Varoquaux, Fabrice

AU - Delorme, Valérie

AU - Lancelin, Jean Marc

AU - Delseny, Michel

AU - Gallois, Patrick

PY - 2011/3/16

Y1 - 2011/3/16

N2 - Programmed cell death (PCD) has a key role in defence and development of all multicellular organisms. In plants, there is a large gap in our knowledge of the molecular machinery involved at the various stages of PCD, especially the early steps. Here, we identify kiss of death (KOD) encoding a 25-amino-acid peptide that activates a PCD pathway in Arabidopsis thaliana. Two mutant alleles of KOD exhibited a reduced PCD of the suspensor, a single file of cells that support embryo development, and a reduced PCD of root hairs after a 55 °C heat shock. KOD expression was found to be inducible by biotic and abiotic stresses. Furthermore, KOD expression was sufficient to cause death in leaves or seedlings and to activate caspase-like activities. In addition, KOD-induced PCD required light in leaves and was repressed by the PCD-suppressor genes AtBax inhibitor 1 and p35. KOD expression resulted in depolarization of the mitochondrial membrane, placing KOD above mitochondria dysfunction, an early step in plant PCD. A KOD-GFP fusion, however, localized in the cytosol of cells and not mitochondria. © 2011 European Molecular Biology Organization | All Rights Reserved.

AB - Programmed cell death (PCD) has a key role in defence and development of all multicellular organisms. In plants, there is a large gap in our knowledge of the molecular machinery involved at the various stages of PCD, especially the early steps. Here, we identify kiss of death (KOD) encoding a 25-amino-acid peptide that activates a PCD pathway in Arabidopsis thaliana. Two mutant alleles of KOD exhibited a reduced PCD of the suspensor, a single file of cells that support embryo development, and a reduced PCD of root hairs after a 55 °C heat shock. KOD expression was found to be inducible by biotic and abiotic stresses. Furthermore, KOD expression was sufficient to cause death in leaves or seedlings and to activate caspase-like activities. In addition, KOD-induced PCD required light in leaves and was repressed by the PCD-suppressor genes AtBax inhibitor 1 and p35. KOD expression resulted in depolarization of the mitochondrial membrane, placing KOD above mitochondria dysfunction, an early step in plant PCD. A KOD-GFP fusion, however, localized in the cytosol of cells and not mitochondria. © 2011 European Molecular Biology Organization | All Rights Reserved.

KW - BAX inhibitor 1

KW - caspase-like

KW - embryogenesis

KW - p35

U2 - 10.1038/emboj.2011.14

DO - 10.1038/emboj.2011.14

M3 - Article

SN - 0261-4189

VL - 30

SP - 1173

EP - 1183

JO - EMBO Journal

JF - EMBO Journal

IS - 6

ER -

The Arabidopsis peptide kiss of death is an inducer of programmed cell death

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