The Arp2/3 activator WASH regulates α5β1-integrin-mediated invasive migration

Tobias Zech, Simon D J Calaminus, Patrick Caswell, Heather J. Spence, Michael Carnell, Robert H. Insall, Jim Norman, Laura M. Machesky

    Research output: Contribution to journalArticlepeer-review


    The actin cytoskeleton provides scaffolding and physical force to effect fundamental processes such as motility, cytokinesis and vesicle trafficking. The Arp2/3 complex nucleates actin structures and contributes to endocytic vesicle invagination and trafficking away from the plasma membrane. Internalisation and directed recycling of integrins are major driving forces for invasive cell motility and potentially for cancer metastasis. Here, we describe a direct requirement for WASH and Arp2/3-mediated actin polymerisation on the endosomal membrane system for α5β1 integrin recycling. WASH regulates the trafficking of endosomal α5β1 integrin to the plasma membrane and is fundamental for integrin-driven cell morphology changes and integrin-mediated cancer cell invasion. Thus, we implicate WASH and Arp2/3-driven actin nucleation in receptor recycling leading to invasive motility. © 2011. Published by The Company of Biologists Ltd.
    Original languageEnglish
    Pages (from-to)3753-3759
    Number of pages6
    JournalJournal of Cell Science
    Issue number22
    Publication statusPublished - 15 Nov 2011


    • Actin assembly
    • Arp2/3 complex
    • Integrin recycling
    • Invasion
    • Membrane trafficking


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