The Aspergillus niger annexin, anxC3.1 is constitutively expressed and is not essential for protein secretion

Vahid Khalaj; Peter Hey; Lyndsay Smith; Geoffrey D. Robson; Jayne Brookman

doi:10.1016/j.femsle.2004.08.034

The Aspergillus niger annexin, anxC3.1 is constitutively expressed and is not essential for protein secretion

Vahid Khalaj, Peter Hey, Lyndsay Smith, Geoffrey D. Robson, Jayne Brookman

Research output: Contribution to journal › Article › peer-review

Abstract

An annexin, anxC3.1, was isolated and characterised from the industrially important filamentous fungus Aspergillus niger. anxC3.1 is a single copy gene encoding a 506 amino acid predicted protein which contains four annexin repeats. Disruption of the anxC3.1 gene did not lead to any visible changes in phenotype, nor in the levels of secreted protein, nor specifically in glucoamylase production, suggesting no major role in secretion. anxC3.1 expression was found to be unaltered under a variety of conditions such as increased secretion, altered nitrogen source, heat shock, and decreased Ca 2+ levels, indicating that anxC3.1 is constitutively expressed. This is the first reported functional characterisation of a fungal annexin. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	163-169
Number of pages	6
Journal	FEMS microbiology letters
Volume	239
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2004.08.034
Publication status	Published - 1 Oct 2004

Keywords

Annexin
Aspergillus niger
Gene disruption
Secretion

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10.1016/j.femsle.2004.08.034

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title = "The Aspergillus niger annexin, anxC3.1 is constitutively expressed and is not essential for protein secretion",

abstract = "An annexin, anxC3.1, was isolated and characterised from the industrially important filamentous fungus Aspergillus niger. anxC3.1 is a single copy gene encoding a 506 amino acid predicted protein which contains four annexin repeats. Disruption of the anxC3.1 gene did not lead to any visible changes in phenotype, nor in the levels of secreted protein, nor specifically in glucoamylase production, suggesting no major role in secretion. anxC3.1 expression was found to be unaltered under a variety of conditions such as increased secretion, altered nitrogen source, heat shock, and decreased Ca 2+ levels, indicating that anxC3.1 is constitutively expressed. This is the first reported functional characterisation of a fungal annexin. {\textcopyright} 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "Annexin, Aspergillus niger, Gene disruption, Secretion",

author = "Vahid Khalaj and Peter Hey and Lyndsay Smith and Robson, \{Geoffrey D.\} and Jayne Brookman",

year = "2004",

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doi = "10.1016/j.femsle.2004.08.034",

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journal = "FEMS microbiology letters",

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TY - JOUR

T1 - The Aspergillus niger annexin, anxC3.1 is constitutively expressed and is not essential for protein secretion

AU - Khalaj, Vahid

AU - Hey, Peter

AU - Smith, Lyndsay

AU - Robson, Geoffrey D.

AU - Brookman, Jayne

PY - 2004/10/1

Y1 - 2004/10/1

N2 - An annexin, anxC3.1, was isolated and characterised from the industrially important filamentous fungus Aspergillus niger. anxC3.1 is a single copy gene encoding a 506 amino acid predicted protein which contains four annexin repeats. Disruption of the anxC3.1 gene did not lead to any visible changes in phenotype, nor in the levels of secreted protein, nor specifically in glucoamylase production, suggesting no major role in secretion. anxC3.1 expression was found to be unaltered under a variety of conditions such as increased secretion, altered nitrogen source, heat shock, and decreased Ca 2+ levels, indicating that anxC3.1 is constitutively expressed. This is the first reported functional characterisation of a fungal annexin. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

AB - An annexin, anxC3.1, was isolated and characterised from the industrially important filamentous fungus Aspergillus niger. anxC3.1 is a single copy gene encoding a 506 amino acid predicted protein which contains four annexin repeats. Disruption of the anxC3.1 gene did not lead to any visible changes in phenotype, nor in the levels of secreted protein, nor specifically in glucoamylase production, suggesting no major role in secretion. anxC3.1 expression was found to be unaltered under a variety of conditions such as increased secretion, altered nitrogen source, heat shock, and decreased Ca 2+ levels, indicating that anxC3.1 is constitutively expressed. This is the first reported functional characterisation of a fungal annexin. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

KW - Annexin

KW - Aspergillus niger

KW - Gene disruption

KW - Secretion

UR - https://www.scopus.com/pages/publications/4644243786

U2 - 10.1016/j.femsle.2004.08.034

DO - 10.1016/j.femsle.2004.08.034

M3 - Article

SN - 1574-6968

VL - 239

SP - 163

EP - 169

JO - FEMS microbiology letters

JF - FEMS microbiology letters

IS - 1

ER -

The Aspergillus niger annexin, anxC3.1 is constitutively expressed and is not essential for protein secretion

Abstract

Keywords

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