The behaviour of both Listeria monocytogenes and Rat ciliated ependymal cells is altered during their co-culture

Mina J. Fadaee-Shohada; Robert A. Hirst; Andrew Rutman; Ian S. Roberts; Chris O'Callaghan; Peter W. Andrew

doi:10.1371/journal.pone.0010450

The behaviour of both Listeria monocytogenes and Rat ciliated ependymal cells is altered during their co-culture

Mina J. Fadaee-Shohada, Robert A. Hirst, Andrew Rutman, Ian S. Roberts, Chris O'Callaghan, Peter W. Andrew

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis. Principal Findings: Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle). Conclusion: The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA). © 2010 Fadaee-Shohada et al.

Original language	English
Article number	e10450
Journal	PLoS ONE
Volume	5
Issue number	5
DOIs	https://doi.org/10.1371/journal.pone.0010450
Publication status	Published - 2010

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title = "The behaviour of both Listeria monocytogenes and Rat ciliated ependymal cells is altered during their co-culture",

abstract = "Background: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis. Principal Findings: Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle). Conclusion: The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA). {\textcopyright} 2010 Fadaee-Shohada et al.",

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T1 - The behaviour of both Listeria monocytogenes and Rat ciliated ependymal cells is altered during their co-culture

AU - Fadaee-Shohada, Mina J.

AU - Hirst, Robert A.

AU - Rutman, Andrew

AU - Roberts, Ian S.

AU - O'Callaghan, Chris

AU - Andrew, Peter W.

PY - 2010

Y1 - 2010

N2 - Background: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis. Principal Findings: Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle). Conclusion: The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA). © 2010 Fadaee-Shohada et al.

AB - Background: Ciliated ependymal cells line the cerebral ventricles and aqueducts separating the infected CSF from the brain parenchyma in meningitis. Principal Findings: Investigation of the interaction of Listeria monocytogenes with cultured rat brain ependymal cells showed that certain strains reduced the beat frequency of the cilia but all the strains studied significantly reduced the ciliary beat amplitude (the linear distance travelled by the tip of each cilium per beat cycle). Conclusion: The presence of the ependyma caused aggregation of some listeria strains and in some cases extracellular material also was seen in association with bacterial aggregates. These observations were dependent on the expression of genes required for invasion, intracellular survival and listerial cell to cell spread that are regulated by the transcriptional activator, positive regulatory factor A (PrfA). © 2010 Fadaee-Shohada et al.

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DO - 10.1371/journal.pone.0010450

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JO - PLoS ONE

JF - PLoS ONE

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ER -

The behaviour of both Listeria monocytogenes and Rat ciliated ependymal cells is altered during their co-culture

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