The bialaphos resistance gene (bar) plays a role in both self-defense and bialaphos biosynthesis in Streptomyces hydroscopicus

Y. Kumada, H. Anzai, E. Takano, T. Murakami, O. Hara, R. Itoh, S. Imai, A. Satoh, K. Nagaoka

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We inactivated the bialaphos (BA) resistance gene (bar) of a BA producer, Streptomyces hygroscopicus, by the gene replacement technique. The resulting BA-sensitive mutant (Bar-) was able to produce little BA but considerable amount of an intermediate demethylphosphinothricin (DMPT). The Bar- mutant was still able to convert the N-acetyl derivative (AcDMPT) of DMPT to BA. Introduction of normal bar containing plasmid restored both BA resistance and BA biosynthesis to levels as high as the parental BA producer. By contrast, introducing a multi copy glutamine synthetase gene (glnA) into the Bar- mutant restored BA resistance but not BA production. Thus, the bar gene plays a crucial role in both self-defense and a step of BA biosynthesis in the BA-producing S. hydroscopicus.
    Original languageEnglish
    Pages (from-to)1838-1845
    Number of pages7
    JournalJournal of Antibiotics
    Volume41
    Issue number12
    Publication statusPublished - 1988

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