The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C-Cdh1

Angel Herrero-Mendez, Angeles Almeida, Emilio Fernández, Carolina Maestre, Salvador Moncada, Juan P. Bolaños

    Research output: Contribution to journalArticlepeer-review


    Neurons are known to have a lower glycolytic rate than astrocytes and when stressed they are unable to upregulate glycolysis because of low Pfkfb3 (6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase-3) activity. This enzyme generates fructose-2,6-bisphosphate (F2,6P2), the most potent activator of 6-phosphofructo-1-kinase (Pfk1; ref. 4), a master regulator of glycolysis. Here, we show that Pfkfb3 is absent from neurons in the brain cortex and that Pfkfb3 in neurons is constantly subject to proteasomal degradation by the action of the E3 ubiquitin ligase, anaphase-promoting complex/cyclosome (APC/C)-Cdh1. By contrast, astrocytes have low APC/C-Cdh1 activity and therefore Pfkfb3 is present in these cells. Upregulation of Pfkfb3 by either inhibition of Cdh1 or overexpression of Pfkfb3 in neurons resulted in the activation of glycolysis. This, however, was accompanied by a marked decrease in the oxidation of glucose through the pentose phosphate pathway (a metabolic route involved in the regeneration of reduced glutathione) resulting in oxidative stress and apoptotic death. Thus, by actively downregulating glycolysis by APC/C-Cdh1, neurons use glucose to maintain their antioxidant status at the expense of its utilization for bioenergetic purposes.
    Original languageEnglish
    Pages (from-to)747-752
    Number of pages5
    JournalNature Cell Biology
    Issue number6
    Publication statusPublished - 2009


    • Amino Acid Sequence
    • Anaphase-Promoting Complex-Cyclosome
    • Animals
    • Antioxidants/*metabolism
    • Cerebral Cortex/cytology
    • *Energy Metabolism
    • Glucose/metabolism
    • Glycolysis/*physiology
    • Humans
    • Isoenzymes/genetics/metabolism
    • Molecular Sequence Data
    • Mutagenesis, Site-Directed
    • Neurons/cytology/*metabolism
    • Nitric Oxide/metabolism
    • Oxidation-Reduction
    • Pentose Phosphate Pathway/physiology
    • Phosphofructokinase-2/genetics/*metabolism
    • RNA Interference
    • RNA, Small Interfering/genetics/metabolism
    • Rats
    • Rats, Wistar
    • Reactive Oxygen Species/metabolism
    • Sequence Alignment
    • Ubiquitin-Protein Ligase Complexes/*metabolism


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