TY - JOUR
T1 - The carboxypeptidase Y-catalysed hydrolysis of aryl trimethylacetates
T2 - The nature of the deacylation reaction of trimethylacetylcarboxypeptidase Y
AU - Douglas, Kenneth T.
AU - Nakagawa, Yasushi
AU - Kaiser, Thomas
PY - 1975
Y1 - 1975
N2 - The identical kcat values obtained for the trimethylacetyl esters of 4-nitrophenol, 2,4-dinitrophenol, and 2-chloro-4-nitrophenol support the intermediacy of a trimethylacetyl enzyme in the esterase action of yeast carboxypeptidase Y, the deacylation of which depends on the ionization of an acidic species (pKapp = 5·1) with limiting kcat values of 0·9 × 10-2 s-1 and 2·2 × 10-2 s-1 in acidic and basic solutions, respectively.
AB - The identical kcat values obtained for the trimethylacetyl esters of 4-nitrophenol, 2,4-dinitrophenol, and 2-chloro-4-nitrophenol support the intermediacy of a trimethylacetyl enzyme in the esterase action of yeast carboxypeptidase Y, the deacylation of which depends on the ionization of an acidic species (pKapp = 5·1) with limiting kcat values of 0·9 × 10-2 s-1 and 2·2 × 10-2 s-1 in acidic and basic solutions, respectively.
UR - http://www.scopus.com/inward/record.url?scp=37049132221&partnerID=8YFLogxK
U2 - 10.1039/C39750000429
DO - 10.1039/C39750000429
M3 - Article
AN - SCOPUS:37049132221
SN - 0022-4936
SP - 429
EP - 430
JO - Journal of the Chemical Society, Chemical Communications
JF - Journal of the Chemical Society, Chemical Communications
IS - 11
ER -