The clonogen content of murine intestinal crypts: Dependence on radiation dose used in its determination

J. H. Hendry; S. A. Roberts; C. S. Potten

doi:10.2307/3578342

The clonogen content of murine intestinal crypts: Dependence on radiation dose used in its determination

J. H. Hendry, S. A. Roberts, C. S. Potten

Research output: Contribution to journal › Article › peer-review

Abstract

The number of colony-forming (clonogenic) cells in each of the crypts in mouse small intestine was deduced using a two-dose irradiation technique. The number was 7.5 ± 0.8 cells using two equal doses each less than 9 Gy and 38 ± 7 cells using 9 Gy or more per dose. The significant dose dependence could not be accounted for by considerations of intra- or intercrypt variability, or by the factor introduced to correct the sampling frequency for the influence of crypt size. The results suggest that more colony-forming cells may be recruited when the injury is more severe.

Original language	English
Pages (from-to)	115-119
Number of pages	4
Journal	Radiation Research
Volume	132
Issue number	1
DOIs	https://doi.org/10.2307/3578342
Publication status	Published - 1992

Keywords

Animal
CANCER
CELLS
CLONOGEN CONTENT
Colony-Forming Units Assay
cytology
DEPENDENCE
Dose-Response Relationship,Radiation
Intestine,Small
IRRADIATION
Male
methods
MICE
MOUSE
NUMBER
RADIATION
radiation effects
small intestine
Stem Cells
Support,Non-U.S.Gov't

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title = "The clonogen content of murine intestinal crypts: Dependence on radiation dose used in its determination",

abstract = "The number of colony-forming (clonogenic) cells in each of the crypts in mouse small intestine was deduced using a two-dose irradiation technique. The number was 7.5 ± 0.8 cells using two equal doses each less than 9 Gy and 38 ± 7 cells using 9 Gy or more per dose. The significant dose dependence could not be accounted for by considerations of intra- or intercrypt variability, or by the factor introduced to correct the sampling frequency for the influence of crypt size. The results suggest that more colony-forming cells may be recruited when the injury is more severe.",

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author = "Hendry, {J. H.} and Roberts, {S. A.} and Potten, {C. S.}",

note = "UI - 93029232LA - engPT - Journal ArticleDA - 19921120IS - 0033-7587SB - IMCY - UNITED STATESJC - QMP",

year = "1992",

doi = "10.2307/3578342",

language = "English",

volume = "132",

pages = "115--119",

journal = "Radiation Research",

publisher = "Radiation Research Society",

number = "1",

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TY - JOUR

T1 - The clonogen content of murine intestinal crypts: Dependence on radiation dose used in its determination

AU - Hendry, J. H.

AU - Roberts, S. A.

AU - Potten, C. S.

N1 - UI - 93029232LA - engPT - Journal ArticleDA - 19921120IS - 0033-7587SB - IMCY - UNITED STATESJC - QMP

PY - 1992

Y1 - 1992

N2 - The number of colony-forming (clonogenic) cells in each of the crypts in mouse small intestine was deduced using a two-dose irradiation technique. The number was 7.5 ± 0.8 cells using two equal doses each less than 9 Gy and 38 ± 7 cells using 9 Gy or more per dose. The significant dose dependence could not be accounted for by considerations of intra- or intercrypt variability, or by the factor introduced to correct the sampling frequency for the influence of crypt size. The results suggest that more colony-forming cells may be recruited when the injury is more severe.

AB - The number of colony-forming (clonogenic) cells in each of the crypts in mouse small intestine was deduced using a two-dose irradiation technique. The number was 7.5 ± 0.8 cells using two equal doses each less than 9 Gy and 38 ± 7 cells using 9 Gy or more per dose. The significant dose dependence could not be accounted for by considerations of intra- or intercrypt variability, or by the factor introduced to correct the sampling frequency for the influence of crypt size. The results suggest that more colony-forming cells may be recruited when the injury is more severe.

KW - Animal

KW - CANCER

KW - CELLS

KW - CLONOGEN CONTENT

KW - Colony-Forming Units Assay

KW - cytology

KW - DEPENDENCE

KW - Dose-Response Relationship,Radiation

KW - Intestine,Small

KW - IRRADIATION

KW - Male

KW - methods

KW - MICE

KW - MOUSE

KW - NUMBER

KW - RADIATION

KW - radiation effects

KW - small intestine

KW - Stem Cells

KW - Support,Non-U.S.Gov't

U2 - 10.2307/3578342

DO - 10.2307/3578342

M3 - Article

VL - 132

SP - 115

EP - 119

JO - Radiation Research

JF - Radiation Research

IS - 1

ER -

The clonogen content of murine intestinal crypts: Dependence on radiation dose used in its determination

Abstract

Keywords

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