The construction of Streptomyces cyaneus genomic libraries in Escherichia coli is dependent upon the use of Mcr-deficient strains

P. Wang; S. S. Harvey; P. F G Sims; P. Broda

The construction of Streptomyces cyaneus genomic libraries in Escherichia coli is dependent upon the use of Mcr-deficient strains

P. Wang, S. S. Harvey, P. F G Sims, P. Broda

Research output: Contribution to journal › Article › peer-review

Abstract

Streptomyces cyaneus genomic DNA ligated into either λ phage or plasmid vectors was very inefficiently cloned into standard Escherichia coli host strains. However, the same material could be efficiently cloned using Mcr-deficient E. coli strains. These results suggest that the S. cyaneus genome contains 5-methylcytosine residues, some of which occur within the recognition sequences of the E. coli Mcr restriction system.

Original language	English
Pages (from-to)	127-129
Number of pages	2
Journal	Gene
Volume	119
Issue number	1
Publication status	Published - 1992

Keywords

genomic libraries
methylated DNA
phage λ vectors
plasmid vectors
restriction systems

Cite this

@article{1076f49e5e6747b181926a3f6222488c,

title = "The construction of Streptomyces cyaneus genomic libraries in Escherichia coli is dependent upon the use of Mcr-deficient strains",

abstract = "Streptomyces cyaneus genomic DNA ligated into either λ phage or plasmid vectors was very inefficiently cloned into standard Escherichia coli host strains. However, the same material could be efficiently cloned using Mcr-deficient E. coli strains. These results suggest that the S. cyaneus genome contains 5-methylcytosine residues, some of which occur within the recognition sequences of the E. coli Mcr restriction system.",

keywords = "genomic libraries, methylated DNA, phage λ vectors, plasmid vectors, restriction systems",

author = "P. Wang and Harvey, {S. S.} and Sims, {P. F G} and P. Broda",

year = "1992",

language = "English",

volume = "119",

pages = "127--129",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - The construction of Streptomyces cyaneus genomic libraries in Escherichia coli is dependent upon the use of Mcr-deficient strains

AU - Wang, P.

AU - Harvey, S. S.

AU - Sims, P. F G

AU - Broda, P.

PY - 1992

Y1 - 1992

N2 - Streptomyces cyaneus genomic DNA ligated into either λ phage or plasmid vectors was very inefficiently cloned into standard Escherichia coli host strains. However, the same material could be efficiently cloned using Mcr-deficient E. coli strains. These results suggest that the S. cyaneus genome contains 5-methylcytosine residues, some of which occur within the recognition sequences of the E. coli Mcr restriction system.

AB - Streptomyces cyaneus genomic DNA ligated into either λ phage or plasmid vectors was very inefficiently cloned into standard Escherichia coli host strains. However, the same material could be efficiently cloned using Mcr-deficient E. coli strains. These results suggest that the S. cyaneus genome contains 5-methylcytosine residues, some of which occur within the recognition sequences of the E. coli Mcr restriction system.

KW - genomic libraries

KW - methylated DNA

KW - phage λ vectors

KW - plasmid vectors

KW - restriction systems

M3 - Article

SN - 0378-1119

VL - 119

SP - 127

EP - 129

JO - Gene

JF - Gene

IS - 1

ER -

The construction of Streptomyces cyaneus genomic libraries in Escherichia coli is dependent upon the use of Mcr-deficient strains

Abstract

Keywords

Fingerprint

Cite this