The cyclin D1 gene is transcriptionally repressed by caveolin-1

James Hulit, Tal Bash, Maofu Fu, Ferruccio Galbiati, Chris Albanese, Daniel R. Sage, Amnon Schlegel, Jacob Zhurinsky, Michael Shtutman, Avri Ben-Ze'ev, Michael P. Lisanti, Richard G. Pestell

    Research output: Contribution to journalArticlepeer-review


    The cyclin D1 gene encodes the regulatory subunit of the holoenzyme that phosphorylates and inactivates the retinoblastoma pRB protein. Cyclin D1 protein levels are elevated by mitogenic and oncogenic signaling pathways, and antisense mRNA to cyclin D1 inhibits transformation by the ras, neu, and src oncogenes, thus linking cyclin D1 regulation to cellular transformation. Caveolins are the principal protein components of caveolae, vesicular plasma membrane invaginations that also function in signal transduction. We show here that caveolin-1 expression levels inversely correlate with cyclin D1 abundance levels in transformed cells. Expression of antisense caveolin-1 increased cyclin D1 levels, whereas caveolin-1 overexpression inhibited expression of the cyclin D1 gene. Cyclin D1 promoter activity was selectively repressed by caveolin-1, but not by caveolin-3, and this repression required the caveolin-1 N terminus. Maximal inhibition of the cyclin D1 gene promoter by caveolin-1 was dependent on the cyclin D1 promoter T-cell factor/lymphoid enhancer factor-1-binding site between -81 to -73. The T-cell factor/lymphoid enhancer factor sequence was sufficient for repression by caveolin-1. We suggest that transcriptional repression of the cyclin D1 gene may contribute to the inhibition of transformation by caveolin-1.
    Original languageEnglish
    Pages (from-to)21203-21209
    Number of pages6
    JournalJournal of Biological Chemistry
    Issue number28
    Publication statusPublished - 14 Jul 2000


    Dive into the research topics of 'The cyclin D1 gene is transcriptionally repressed by caveolin-1'. Together they form a unique fingerprint.

    Cite this