TY - JOUR
T1 - The deleterious effect of high concentrations of D-glucose requires pro-inflammatory preconditioning
AU - Lafuente, Nuria
AU - Matesanz, Nuria
AU - Azcutia, Verónica
AU - Romacho, Tania
AU - Nevado, Julián
AU - Rodríguez-Mañas, Leocadio
AU - Moncada, Salvador
AU - Peiró, Concepción
AU - Sánchez-Ferrer, Carlos F.
N1 - Lafuente, Nuria Matesanz, Nuria Azcutia, Veronica Romacho, Tania Nevado, Julian Rodriguez-Manas, Leocadio Moncada, Salvador Peiro, Concepcion Sanchez-Ferrer, Carlos F England J Hypertens. 2008 Mar;26(3):478-85. doi: 10.1097/HJH.0b013e3282f331fb.
PY - 2008/3
Y1 - 2008/3
N2 - OBJECTIVES: The present study investigated whether high concentrations of D-glucose can trigger pro-inflammatory mechanisms in human aortic smooth muscle cells. METHODS: The expression and/or the activity of inducible nitric oxide synthase (iNOS), the extracellular signal-regulated kinase (ERK) 1/2 and nuclear factor (NF)-κB were studied in cultured human aortic smooth muscle cells (HASMC) in response to increasing concentrations of D-glucose and/or the inflammatory cytokine interleukin (IL)-1β. RESULTS: Increasing D-glucose in the medium from 5.5 to 22 mmol/l had no effect on any of these parameters. However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1β (2.5 and 5 ng/ml), as well as the IL-1β-induced activation of both ERK 1/2 and NF-κB. D-glucose also enhanced, concentration-dependently, the expression and activity of iNOS induced by co-incubation with IL-1β (10 ng/ml). Pretreatment with IL-1β sensitized the cells to the subsequent effects of high D-glucose. CONCLUSIONS: The results indicate that high concentrations of D-glucose exacerbate the pro-inflammatory effects of IL-1β. We suggest that the observed association between inflammation and diabetes is the result of elevated D-glucose enhancing a pre-existing inflammatory condition, rather than a direct effect of D-glucose on the production of inflammatory mediators. © 2008 Lippincott Williams & Wilkins, Inc.
AB - OBJECTIVES: The present study investigated whether high concentrations of D-glucose can trigger pro-inflammatory mechanisms in human aortic smooth muscle cells. METHODS: The expression and/or the activity of inducible nitric oxide synthase (iNOS), the extracellular signal-regulated kinase (ERK) 1/2 and nuclear factor (NF)-κB were studied in cultured human aortic smooth muscle cells (HASMC) in response to increasing concentrations of D-glucose and/or the inflammatory cytokine interleukin (IL)-1β. RESULTS: Increasing D-glucose in the medium from 5.5 to 22 mmol/l had no effect on any of these parameters. However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1β (2.5 and 5 ng/ml), as well as the IL-1β-induced activation of both ERK 1/2 and NF-κB. D-glucose also enhanced, concentration-dependently, the expression and activity of iNOS induced by co-incubation with IL-1β (10 ng/ml). Pretreatment with IL-1β sensitized the cells to the subsequent effects of high D-glucose. CONCLUSIONS: The results indicate that high concentrations of D-glucose exacerbate the pro-inflammatory effects of IL-1β. We suggest that the observed association between inflammation and diabetes is the result of elevated D-glucose enhancing a pre-existing inflammatory condition, rather than a direct effect of D-glucose on the production of inflammatory mediators. © 2008 Lippincott Williams & Wilkins, Inc.
KW - High glucose concentration
KW - Human vascular smooth muscle
KW - Vascular inflammation
U2 - 10.1097/HJH.0b013e3282f331fb
DO - 10.1097/HJH.0b013e3282f331fb
M3 - Article
SN - 0263-6352
VL - 26
SP - 478
EP - 485
JO - Journal of hypertension
JF - Journal of hypertension
IS - 3
ER -