The development of a bioluminescence assay to compare the efficacy of biocides incorporated into plasticised PVC

Catherine Jones, Geoffrey D. Robson, Malcolm Greenhaulgh, Ian Eastwood, Pauline S. Handley

    Research output: Contribution to journalArticlepeer-review

    Abstract

    A bioluminescence assay was developed using the expression of the luxAB genes in Pseudomonas veronii to allow the efficacy of biocides incorporated into plasticised polyvinylchloride (pPVC) to be determined in situ. A maximum number of cells was found to adhere to the surface after 18h as measured by bioluminescence, radiolabelling and viable cell counts. A positive correlation was found between the level of bioluminescence and numbers of viable cells attached to the pPVC. When the biocide 10, 10-oxybisphenoxyarsine (OBPA) was incorporated into the pPVC, both bioluminescence and viable cell number were reduced by ca 60% at a concentration of 750 ppm and by >99% at 2250 ppm. When the biocide 2,3,5,6-tetrachloro-4-(methylsulphonyl)pyridine (TCMP) was incorporated into the pPVC, no reduction in viability or bioluminescence was seen after 18 h. However, over a period of 72h at a concentration of 2250 ppm TCMP, both viable cell number and bioluminescence decreased steadily after 36h until after 72h, both bioluminescence and viable cell counts were less than 1% of the initial values. The viability of attached cells can therefore be measured in situ in a sensitive real-time assay by measuring bioluminescence allowing the efficacy of biocides incorporated into plastics to be compared.
    Original languageEnglish
    Pages (from-to)21-27
    Number of pages6
    JournalBiofouling
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Bioluminescence
    • Efficacy
    • LuxAB genes
    • Pseudomonas veronii

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