The dynamic architecture of the metabolic switch in Streptomyces coelicolor

Kay Nieselt, Florian Battke, Alexander Herbig, Per Bruheim, Alexander Wentzel, Øyvind M. Jakobsen, Håvard Sletta, Mohammad T. Alam, Maria E. Merlo, Jonathan Moore, Walid A M Omara, Edward R. Morrissey, Miguel A. Juarez-Hermosillo, Antonio Rodríguez-García, Merle Nentwich, Louise Thomas, Mudassar Iqbal, Roxane Legaie, William H. Gaze, Gregory L. ChallisRitsert C. Jansen, Lubbert Dijkhuizen, David A. Rand, David L. Wild, Michael Bonin, Jens Reuther, Wolfgang Wohlleben, Margaret C M Smith, Nigel J. Burroughs, Juan F. Martín, David A. Hodgson, Eriko Takano, Rainer Breitling, Trond E. Ellingsen, Elizabeth M H Wellington

    Research output: Contribution to journalArticlepeer-review


    Background: During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major
    metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns
    during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermentergrown
    Results: Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching
    events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before
    the classically defined transition phase where the switch from primary to secondary metabolism was expected. The
    main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are delayed
    relative to the metabolic rearrangements. Furthermore, global variation in morphogenesis genes indicates an
    involvement of cell differentiation pathways in the decision phase leading up to the commitment to antibiotic
    Conclusions: Our study provides the first detailed insights into the complex sequence of early regulatory events
    during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future
    attempts at engineering antibiotic production in a biotechnological setting
    Original languageEnglish
    Pages (from-to)1-9
    Number of pages9
    JournalBMC Genomics
    Issue number10
    Publication statusPublished - 6 Jan 2010


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