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Methods and Results: In vitro study: Peripheral blood mononuclear cells (PBMC) were isolated from healthy volunteers and histone H2AX phosphorylation (γ-H2AX) expression, leukocyte counts and functional parameters were quantified using flow cytometry under the following conditions: 1) immediately following PBMC isolation, 2) after standing on the benchside as a temperature and time control, 3) after a standard CMR scan. In vivo study: Blood samples were taken from 64 consecutive consenting patients immediately before and after a standard clinical scan. Samples were analysed for γ-H2AX expression and leukocyte counts.
CMR was not associated with a significant change in γ-H2AX expression in vitro or in vivo, although there were significant inter-patient variations. In vitro cell integrity and function did not change with CMR. There was a significant reduction in circulating T cells in vivo following CMR.
Conclusion: 1.5T CMR was not associated with DNA damage in vitro or in vivo. γ-H2AX expression varied markedly between individuals, therefore small studies using γ-H2AX as a marker of DNA damage should be interpreted with caution. CMR was not associated with loss of leukocyte viability or function in vitro. CMR was associated with a statistically significant reduction in viable leukocytes in vivo.
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- 1 Finished
1/09/16 → 30/09/22