The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes

J. N.A. Tettey; G. G. Skellern; J. M. Midgley; M. H. Grant; A. R. Pitt

doi:10.1016/S0009-2797(99)00127-1

The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes

J. N.A. Tettey, G. G. Skellern^*, J. M. Midgley, M. H. Grant, A. R. Pitt

^*Corresponding author for this work

Analytical, Measurements and Physical Chemistry

University of Strathclyde

Research output: Contribution to journal › Article › peer-review

Abstract

The metabolism of ethidium bromide by isolated rat hepatocytes is significantly enhanced by pre-treatment of animals with phenobarbitone (PB) and 3-methylcholanthrene (3-MC). Pre-treatment with PB and 3-MC results in a 2.5- and 1.5-fold increase, respectively in the amount of the principal metabolite, ethidium 8-N-glucuronide, compared with that formed by hepatocytes from untreated rats. The formation of ethidium 3-N-glucuronide is not enhanced by pre-treatment with either PB or 3-MC. Two new metabolites, hydroxyethidium glucuronide and a transient unidentified species, have been detected by HPLC and are formed only by hepatocytes from animals pre-treated with 3-MC.

Original language	English
Pages (from-to)	105-115
Number of pages	11
Journal	Chemico-Biological Interactions
Volume	123
Issue number	2
DOIs	https://doi.org/10.1016/S0009-2797(99)00127-1
Publication status	Published - 30 Nov 1999

Keywords

Ethidium bromide
Hepatocytes
Inducing agents
Metabolism
N-Glucuronidation

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10.1016/S0009-2797(99)00127-1

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title = "The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes",

abstract = "The metabolism of ethidium bromide by isolated rat hepatocytes is significantly enhanced by pre-treatment of animals with phenobarbitone (PB) and 3-methylcholanthrene (3-MC). Pre-treatment with PB and 3-MC results in a 2.5- and 1.5-fold increase, respectively in the amount of the principal metabolite, ethidium 8-N-glucuronide, compared with that formed by hepatocytes from untreated rats. The formation of ethidium 3-N-glucuronide is not enhanced by pre-treatment with either PB or 3-MC. Two new metabolites, hydroxyethidium glucuronide and a transient unidentified species, have been detected by HPLC and are formed only by hepatocytes from animals pre-treated with 3-MC.",

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author = "Tettey, {J. N.A.} and Skellern, {G. G.} and Midgley, {J. M.} and Grant, {M. H.} and Pitt, {A. R.}",

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T1 - The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes

AU - Tettey, J. N.A.

AU - Skellern, G. G.

AU - Midgley, J. M.

AU - Grant, M. H.

AU - Pitt, A. R.

PY - 1999/11/30

Y1 - 1999/11/30

N2 - The metabolism of ethidium bromide by isolated rat hepatocytes is significantly enhanced by pre-treatment of animals with phenobarbitone (PB) and 3-methylcholanthrene (3-MC). Pre-treatment with PB and 3-MC results in a 2.5- and 1.5-fold increase, respectively in the amount of the principal metabolite, ethidium 8-N-glucuronide, compared with that formed by hepatocytes from untreated rats. The formation of ethidium 3-N-glucuronide is not enhanced by pre-treatment with either PB or 3-MC. Two new metabolites, hydroxyethidium glucuronide and a transient unidentified species, have been detected by HPLC and are formed only by hepatocytes from animals pre-treated with 3-MC.

AB - The metabolism of ethidium bromide by isolated rat hepatocytes is significantly enhanced by pre-treatment of animals with phenobarbitone (PB) and 3-methylcholanthrene (3-MC). Pre-treatment with PB and 3-MC results in a 2.5- and 1.5-fold increase, respectively in the amount of the principal metabolite, ethidium 8-N-glucuronide, compared with that formed by hepatocytes from untreated rats. The formation of ethidium 3-N-glucuronide is not enhanced by pre-treatment with either PB or 3-MC. Two new metabolites, hydroxyethidium glucuronide and a transient unidentified species, have been detected by HPLC and are formed only by hepatocytes from animals pre-treated with 3-MC.

KW - Ethidium bromide

KW - Hepatocytes

KW - Inducing agents

KW - Metabolism

KW - N-Glucuronidation

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JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

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ER -

The effect of inducing agents on the metabolism of ethidium bromide by isolated rat hepatocytes

Abstract

Keywords

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