The effect of receptor clustering on vesicle-vesicle adhesion

Robert J. Mart; Kwan Ping Liem; Xi Wang; Simon J. Webb

doi:10.1021/ja0657612

The effect of receptor clustering on vesicle-vesicle adhesion

Robert J. Mart, Kwan Ping Liem, Xi Wang, Simon J. Webb

Research output: Contribution to journal › Article › peer-review

Abstract

As part of our studies into how the localization of cell adhesion molecules into lipid rafts may affect cell adhesion, we developed Cu(1), a synthetic copper(iminodiacetate)-capped receptor able to phase separate from fluid phospholipid bilayers. The extent to which Cu(1) clustered into adhesive patches on the surface of vesicles could be controlled by changing vesicle composition. Extensive receptor phase separation significantly enhanced vesicle-vesicle adhesion; only vesicles with adhesive patches (blue fluorescence) adhered to their conjugate histidine-coated vesicles (red fluorescence) to form large vesicle aggregates (shown). Copyright © 2006 American Chemical Society.

Original language	English
Pages (from-to)	14462-14463
Number of pages	1
Journal	Journal of the American Chemical Society
Volume	128
Issue number	45
DOIs	https://doi.org/10.1021/ja0657612
Publication status	Published - 15 Nov 2006

Keywords

Membrane (bilayer
effect of receptor clustering on vesicle-vesicle adhesion)
Adhesion (cell adhesion
Organelle (coated vesicle, histidine-coated
Phase separation (effect of receptor clustering on vesicle-vesicle adhesion)
Receptors Role: BSU (Biological study, unclassified), BIOL (Biological study) (effect of receptor clustering on vesicle-vesicle adhesion)
Cell adhesion molecules
Phospholipids Role: BSU (Biological study, unclassified), PRP (Properties), BIOL (Biological study) (effect of receptor clustering on vesicle-vesicle adhesion)
Molecular association (vesicle-vesicle
receptor vesicle adhesion phospholipid bilayer

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10.1021/ja0657612

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title = "The effect of receptor clustering on vesicle-vesicle adhesion",

abstract = "As part of our studies into how the localization of cell adhesion molecules into lipid rafts may affect cell adhesion, we developed Cu(1), a synthetic copper(iminodiacetate)-capped receptor able to phase separate from fluid phospholipid bilayers. The extent to which Cu(1) clustered into adhesive patches on the surface of vesicles could be controlled by changing vesicle composition. Extensive receptor phase separation significantly enhanced vesicle-vesicle adhesion; only vesicles with adhesive patches (blue fluorescence) adhered to their conjugate histidine-coated vesicles (red fluorescence) to form large vesicle aggregates (shown). Copyright {\textcopyright} 2006 American Chemical Society.",

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author = "Mart, {Robert J.} and Liem, {Kwan Ping} and Xi Wang and Webb, {Simon J.}",

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year = "2006",

month = nov,

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doi = "10.1021/ja0657612",

language = "English",

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T1 - The effect of receptor clustering on vesicle-vesicle adhesion

AU - Mart, Robert J.

AU - Liem, Kwan Ping

AU - Wang, Xi

AU - Webb, Simon J.

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PY - 2006/11/15

Y1 - 2006/11/15

N2 - As part of our studies into how the localization of cell adhesion molecules into lipid rafts may affect cell adhesion, we developed Cu(1), a synthetic copper(iminodiacetate)-capped receptor able to phase separate from fluid phospholipid bilayers. The extent to which Cu(1) clustered into adhesive patches on the surface of vesicles could be controlled by changing vesicle composition. Extensive receptor phase separation significantly enhanced vesicle-vesicle adhesion; only vesicles with adhesive patches (blue fluorescence) adhered to their conjugate histidine-coated vesicles (red fluorescence) to form large vesicle aggregates (shown). Copyright © 2006 American Chemical Society.

AB - As part of our studies into how the localization of cell adhesion molecules into lipid rafts may affect cell adhesion, we developed Cu(1), a synthetic copper(iminodiacetate)-capped receptor able to phase separate from fluid phospholipid bilayers. The extent to which Cu(1) clustered into adhesive patches on the surface of vesicles could be controlled by changing vesicle composition. Extensive receptor phase separation significantly enhanced vesicle-vesicle adhesion; only vesicles with adhesive patches (blue fluorescence) adhered to their conjugate histidine-coated vesicles (red fluorescence) to form large vesicle aggregates (shown). Copyright © 2006 American Chemical Society.

KW - Membrane (bilayer

KW - effect of receptor clustering on vesicle-vesicle adhesion)

KW - Adhesion (cell adhesion

KW - Organelle (coated vesicle, histidine-coated

KW - Phase separation (effect of receptor clustering on vesicle-vesicle adhesion)

KW - Receptors Role: BSU (Biological study, unclassified), BIOL (Biological study) (effect of receptor clustering on vesicle-vesicle adhesion)

KW - Cell adhesion molecules

KW - Phospholipids Role: BSU (Biological study, unclassified), PRP (Properties), BIOL (Biological study) (effect of receptor clustering on vesicle-vesicle adhesion)

KW - Molecular association (vesicle-vesicle

KW - receptor vesicle adhesion phospholipid bilayer

U2 - 10.1021/ja0657612

DO - 10.1021/ja0657612

M3 - Article

SN - 0002-7863

VL - 128

SP - 14462

EP - 14463

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 45

ER -

The effect of receptor clustering on vesicle-vesicle adhesion

Abstract

Keywords

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