A human embryonic lung cell line (AP-9) and two malignant cell lines SKL-2 (lymphoblastic leukaemia cells) and EB-2 (Burkitt lymphoma) were used to measure hormonally stimulated cyclic adenosine 3′5′-monophosphate (cAMP) levels. Isoprenaline and prostaglandin E1 (PGE1) produced much higher levels of cAMP in AP-9 than in either of the malignant cell lines. The local anaesthetic tetracaine was used as an agent to alter membrane mobility, and the response of hormonally stimulated cAMP varied in the three cell lines. However, the action of tetracaine on isoprenaline and prostaglandin E1 (PGE1) stimulation was similar in any given cell line. In AP-9 cells 0.5 mM tetracaine produced a peak in hormonally stimulated cAMP levels although higher concentrations caused inhibition. In contrast, tetracaine produced an enhancement of hormonal stimulation in SKL-2 cells and a combined response in EB-2 cells. These results are discussed with regard to recent evidence proposing that tetracaine affects cytoskeletal organisation. © 1977.
|Number of pages||5|
|Journal||Experimental Cell Research|
|Publication status||Published - Jun 1977|