The effects of metabolic inhibition on intracellular pH and Ca

S. C. O'Neill, M. Valdeolmillos, G. L. Smith, D. A. Eisner

    Research output: Contribution to journalArticlepeer-review


    We have investigated the effects of inhibiting aerobic and/or anaerobic metabolism on contraction, intracellular calcium and pH in single rat ventricular myocytes. Inhibition of aerobic metabolism alone (with CN) had little effect on these variables. However, if anaerobic glycolysis was also inhibited, then the application of CN decreased systolic [Ca3+]i and increased diastolic [Ca2+]i. There was also a development of a diastolic contracture which lagged behind the increase of diastolic [Ca2+]i. These events were accompanied by an intracellular acidosis. The acidosis was shown to depress contraction and perhaps to account for the fact that diastolic [Ca2+]i increased before the contracture. © 1989 Kluwer Academic Publishers.
    Original languageEnglish
    Pages (from-to)199-203
    Number of pages4
    JournalMolecular and Cellular Biochemistry
    Issue number2
    Publication statusPublished - Sept 1989


    • acidosis
    • intracellular pH
    • metabolism
    • rat ventricular myocytes


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