The Elk-1 ETS-domain transcription factor contains a mitogen-activated protein kinase targeting motif

Shen Hsi Yang, Paula R. Yates, Alan J. Whitmarsh, Roger J. Davis, Andrew D. Sharrocks

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The phosphorylation of transcription factors by mitogen-activated protein kinases (MAP) is a pivotal event in the cellular response to the activation of MAP kinase signal transduction pathways. Mitogenic and stress stimuli activate different pathways and lead to the activation of distinct groups of target proteins. EIk-1 is targeted by three distinct MAP kinase pathways. In this study, we demonstrate that the MAP kinase ERK2 is targeted to Elk-1 by a domain which is distinct from, and located N-terminally to, its phosphoacceptor motifs. Targeting via this domain is essential for the efficient and rapid phosphorylation of Elk-1 in vitro and full and rapid activation in vivo. Specific residues involved in ERK targeting have been identified. Our data indicate that the targeting of different classes of MAP kinases to their nuclear substrates may be a common mechanism to increase the specificity and efficiency of this signal transduction pathway.
    Original languageEnglish
    Pages (from-to)710-720
    Number of pages10
    JournalMolecular and Cellular Biology
    Volume18
    Issue number2
    Publication statusPublished - Feb 1998

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