The ETS domain transcription factor Elk-1 contains a novel class of repression domain

Shen Hsi Yang, Donna C. Bumpass, Neil D. Perkins, Andrew D. Sharrocks

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The ETS domain transcription factor Elk-1 serves as an integration point for different mitogen-activated protein (MAP) kinase pathways. Phosphorylation of Elk-1 by MAP kinases triggers its activation. However, while the activation process is well understood, its downregulation-inactivation is less well characterized. The ETS DNA-binding domain plays a role in the downregulation of Elk-dependent promoter activity following mitogenic activation by recruiting the mSin3A-HDAC complex. Here we have identified a novel evolutionarily conserved repression domain in Elk-1, termed the R motif, which serves to reduce the basal transcriptional activity of Elk-1 and dampen its response to mitogenic signals. This domain is highly potent and portable and can repress transcription in trans. The R motif is related to the CRD1 repression domain in p300 and can functionally replace this domain and confer p21waf1/cip1 inducibility on p300. However, the R motif acts in a context-dependent manner and is not p21waf1/cip1 responsive in Elk-1. Thus, the Elk-1 R motif and the p300 CRD1 motif represent a new class of repression domains that are regulated in a context-dependent manner.
    Original languageEnglish
    Pages (from-to)5036-5046
    Number of pages10
    JournalMolecular and Cellular Biology
    Volume22
    Issue number14
    DOIs
    Publication statusPublished - 2002

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