The expression of mucin genes and the presence of mucin gene products in the equine endometrium

Eva Maischberger; Carolyn A. Cummins; Eamonn Fitzpatrick; Mary E. Gallagher; Sheila Worrall; Karine Rousseau; David J. Thornton; Wim G. Meijer; Raúl Miranda-CasoLuengo; Vivienne E. Duggan; Stephen D. Carrington; Jane A. Irwin; Colm J. Reid

doi:10.1016/j.rvsc.2013.03.012

The expression of mucin genes and the presence of mucin gene products in the equine endometrium

Eva Maischberger, Carolyn A. Cummins, Eamonn Fitzpatrick, Mary E. Gallagher, Sheila Worrall, Karine Rousseau, David J. Thornton, Wim G. Meijer, Raúl Miranda-CasoLuengo, Vivienne E. Duggan, Stephen D. Carrington, Jane A. Irwin, Colm J. Reid

Research output: Contribution to journal › Article › peer-review

Abstract

In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B). © 2013 Elsevier Ltd.

Original language	English
Pages (from-to)	169-175
Number of pages	6
Journal	Research in Veterinary Science
Volume	95
Issue number	1
DOIs	https://doi.org/10.1016/j.rvsc.2013.03.012
Publication status	Published - Aug 2013

Keywords

Equine female reproductive tract
Equine mucin genes
In situ hybridisation
Oestrous cycle
Reverse transcriptase polymerase chain reaction

Access to Document

10.1016/j.rvsc.2013.03.012

Biological Mass Spectrometry (BioMS) Facility
Knight, D. (Platform Lead), Warwood, S. (Senior Technical Specialist), Selley, J. (Technical Specialist), Taylor, G. (Technical Specialist), Fullwood, P. (Technical Specialist), Keevill, E.-J. (Senior Technician) & Allsey, J. (Technician)
FBMH Platform Sciences, Enabling Technologies & Infrastructure
Facility/equipment: Facility

Cite this

Maischberger, E., Cummins, C. A., Fitzpatrick, E., Gallagher, M. E., Worrall, S., Rousseau, K., Thornton, D. J., Meijer, W. G., Miranda-CasoLuengo, R., Duggan, V. E., Carrington, S. D., Irwin, J. A., & Reid, C. J. (2013). The expression of mucin genes and the presence of mucin gene products in the equine endometrium. Research in Veterinary Science, 95(1), 169-175. https://doi.org/10.1016/j.rvsc.2013.03.012

@article{03607a3e1c2745de95b4e490a5f08d79,

title = "The expression of mucin genes and the presence of mucin gene products in the equine endometrium",

abstract = "In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B). {\textcopyright} 2013 Elsevier Ltd.",

keywords = "Equine female reproductive tract, Equine mucin genes, In situ hybridisation, Oestrous cycle, Reverse transcriptase polymerase chain reaction",

author = "Eva Maischberger and Cummins, {Carolyn A.} and Eamonn Fitzpatrick and Gallagher, {Mary E.} and Sheila Worrall and Karine Rousseau and Thornton, {David J.} and Meijer, {Wim G.} and Ra{\'u}l Miranda-CasoLuengo and Duggan, {Vivienne E.} and Carrington, {Stephen D.} and Irwin, {Jane A.} and Reid, {Colm J.}",

year = "2013",

month = aug,

doi = "10.1016/j.rvsc.2013.03.012",

language = "English",

volume = "95",

pages = "169--175",

journal = "Research in Veterinary Science",

issn = "0034-5288",

publisher = "Elsevier BV",

number = "1",

}

Maischberger, E, Cummins, CA, Fitzpatrick, E, Gallagher, ME, Worrall, S, Rousseau, K, Thornton, DJ, Meijer, WG, Miranda-CasoLuengo, R, Duggan, VE, Carrington, SD, Irwin, JA & Reid, CJ 2013, 'The expression of mucin genes and the presence of mucin gene products in the equine endometrium', Research in Veterinary Science, vol. 95, no. 1, pp. 169-175. https://doi.org/10.1016/j.rvsc.2013.03.012

TY - JOUR

T1 - The expression of mucin genes and the presence of mucin gene products in the equine endometrium

AU - Maischberger, Eva

AU - Cummins, Carolyn A.

AU - Fitzpatrick, Eamonn

AU - Gallagher, Mary E.

AU - Worrall, Sheila

AU - Rousseau, Karine

AU - Thornton, David J.

AU - Meijer, Wim G.

AU - Miranda-CasoLuengo, Raúl

AU - Duggan, Vivienne E.

AU - Carrington, Stephen D.

AU - Irwin, Jane A.

AU - Reid, Colm J.

PY - 2013/8

Y1 - 2013/8

N2 - In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B). © 2013 Elsevier Ltd.

AB - In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B). © 2013 Elsevier Ltd.

KW - Equine female reproductive tract

KW - Equine mucin genes

KW - In situ hybridisation

KW - Oestrous cycle

KW - Reverse transcriptase polymerase chain reaction

U2 - 10.1016/j.rvsc.2013.03.012

DO - 10.1016/j.rvsc.2013.03.012

M3 - Article

C2 - 23583093

SN - 0034-5288

VL - 95

SP - 169

EP - 175

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 1

ER -

The expression of mucin genes and the presence of mucin gene products in the equine endometrium

Abstract

Keywords

Access to Document

Fingerprint

Equipment

Biological Mass Spectrometry (BioMS) Facility

Cite this