The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations

Christopher J. Kershaw; Nigel L. Brown; Chrystala Constantinidou; Mala D. Patel; Jon L. Hobman

doi:10.1099/mic.0.27650-0

The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations

Christopher J. Kershaw, Nigel L. Brown, Chrystala Constantinidou, Mala D. Patel, Jon L. Hobman

Research output: Contribution to journal › Article › peer-review

Abstract

The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 μM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response. © 2005 SGM.

Original language	English
Pages (from-to)	1187-1198
Number of pages	11
Journal	Microbiology
Volume	151
Issue number	4
DOIs	https://doi.org/10.1099/mic.0.27650-0
Publication status	Published - Apr 2005

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title = "The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations",

abstract = "The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 μM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response. {\textcopyright} 2005 SGM.",

author = "Kershaw, {Christopher J.} and Brown, {Nigel L.} and Chrystala Constantinidou and Patel, {Mala D.} and Hobman, {Jon L.}",

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T1 - The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations

AU - Kershaw, Christopher J.

AU - Brown, Nigel L.

AU - Constantinidou, Chrystala

AU - Patel, Mala D.

AU - Hobman, Jon L.

N1 - Times Cited: 48 Part 4

PY - 2005/4

Y1 - 2005/4

N2 - The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 μM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response. © 2005 SGM.

AB - The gene expression profile of Escherichia coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole-genome oligonucleotide microarrays. At 750 μM copper-glycine, the expression of both the cue and cus copper-export systems is evident. At near-lethal copper concentrations (2 mM copper-glycine), the expression of these two regulons increases significantly. Other regulons with increased transcription in response to elevated concentrations of copper-glycine include those for the superoxide stress response, iron homeostasis, and envelope stress. Furthermore, a variety of ORFs with decreased expression in response to increased copper-glycine has been identified, including the zinc ABC transporter and genes involved in the chemotactic response. © 2005 SGM.

U2 - 10.1099/mic.0.27650-0

DO - 10.1099/mic.0.27650-0

M3 - Article

SN - 1350-0872

VL - 151

SP - 1187

EP - 1198

JO - Microbiology

JF - Microbiology

IS - 4

ER -

The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations

Abstract

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