The fetal mouse metatarsal bone explant as a model of angiogenesis.

Weihua Song; Chee Wai Fhu; Koon Hwee Ang; Cheng Hao Liu; Nurul Azizah Binte Johari; Daniel Lio; Sabu Abraham; Wanjin Hong; Stephen E Moss; John Greenwood; Xiaomeng Wang

doi:10.1038/nprot.2015.097

The fetal mouse metatarsal bone explant as a model of angiogenesis.

Weihua Song, Chee Wai Fhu, Koon Hwee Ang, Cheng Hao Liu, Nurul Azizah Binte Johari, Daniel Lio, Sabu Abraham, Wanjin Hong, Stephen E Moss, John Greenwood, Xiaomeng Wang

Research output: Contribution to journal › Article › peer-review

Abstract

The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel growth, and the mechanisms underpinning this process, in a multicellular microenvironment that drives the formation of a robust and complex vascular network in the absence of exogenous growth factors. By labeling different constituents of the vascular structure, it is possible to perform 3D rendering of the spatial interplay between different cellular components and to carry out quantitative analysis of vessel outgrowth. High-resolution imaging permits the visualization of fine structural and cellular details. As the assay involves the use of fetal tissues, it is possible to follow new blood vessel formation in genetically modified mice that are perinatally lethal. The entire process takes 9-13 d. A detailed description of how to set up and perform the assay is described here.

Original language	English
Journal	Nature protocols
Volume	10
Issue number	10
DOIs	https://doi.org/10.1038/nprot.2015.097
Publication status	Published - Oct 2015

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title = "The fetal mouse metatarsal bone explant as a model of angiogenesis.",

abstract = "The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel growth, and the mechanisms underpinning this process, in a multicellular microenvironment that drives the formation of a robust and complex vascular network in the absence of exogenous growth factors. By labeling different constituents of the vascular structure, it is possible to perform 3D rendering of the spatial interplay between different cellular components and to carry out quantitative analysis of vessel outgrowth. High-resolution imaging permits the visualization of fine structural and cellular details. As the assay involves the use of fetal tissues, it is possible to follow new blood vessel formation in genetically modified mice that are perinatally lethal. The entire process takes 9-13 d. A detailed description of how to set up and perform the assay is described here.",

author = "Weihua Song and Fhu, {Chee Wai} and Ang, {Koon Hwee} and Liu, {Cheng Hao} and Johari, {Nurul Azizah Binte} and Daniel Lio and Sabu Abraham and Wanjin Hong and Moss, {Stephen E} and John Greenwood and Xiaomeng Wang",

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AU - Song, Weihua

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AU - Ang, Koon Hwee

AU - Liu, Cheng Hao

AU - Johari, Nurul Azizah Binte

AU - Lio, Daniel

AU - Abraham, Sabu

AU - Hong, Wanjin

AU - Moss, Stephen E

AU - Greenwood, John

AU - Wang, Xiaomeng

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AB - The mouse fetal metatarsal provides a unique tool for studying angiogenesis. In comparison with other commonly used in vitro or ex vivo angiogenesis assays, vessel outgrowth from mouse fetal metatarsals is more representative of sprouting angiogensis in vivo. It allows the analysis of blood vessel growth, and the mechanisms underpinning this process, in a multicellular microenvironment that drives the formation of a robust and complex vascular network in the absence of exogenous growth factors. By labeling different constituents of the vascular structure, it is possible to perform 3D rendering of the spatial interplay between different cellular components and to carry out quantitative analysis of vessel outgrowth. High-resolution imaging permits the visualization of fine structural and cellular details. As the assay involves the use of fetal tissues, it is possible to follow new blood vessel formation in genetically modified mice that are perinatally lethal. The entire process takes 9-13 d. A detailed description of how to set up and perform the assay is described here.

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The fetal mouse metatarsal bone explant as a model of angiogenesis.

Abstract

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