The GADD45a-GFP greenscreen HC assay

Richard M. Walmsley, Matthew Tate

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Mutagens, clastogens, and aneugens cause increased expression of the human GADD45a gene. This has been exploited in the GreenScreen HC genotoxicity assay in which the gene's expression is linked to the expression of green fluorescent protein (GFP). The host for the reporter construct is the human lymphoblastoid cell line TK6. It was chosen for its growth as a cell suspension, which allows simple pipette transfers, and for its wild-type p53 competent status. P53 is required for proper GADD45a expression, and more generally for genome stability. TK6 is a karyotypically stable cell line. The GreenScreen assays were designed to facilitate screening, and this is reflected in its microplate format and low compound requirement. Protocols are available for testing with and without S9 as a source of exogenous metabolic activation. Data is collected either spectrophotometrically or by flow cytometry, and a simple spreadsheet converts raw data into dose-response curves, and provides a statistically significant positive or negative result. Extensive validation has demonstrated that in contrast to other in vitro mammalian genotoxicity assays, the GADD45a assays have both high sensitivity and specificity-they very rarely produce misleading positive results. © 2012 Springer Science+Business Media, LLC.
    Original languageEnglish
    Pages (from-to)231-250
    Number of pages19
    JournalMethods in Molecular Biology
    Volume817
    DOIs
    Publication statusPublished - 2012

    Keywords

    • GADD45a
    • GADD45a-GFP
    • Genotoxicity
    • GreenScreen HC
    • Screening

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