The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to Iq21-q23 and RFLP analysis

I Tepler; C C Morton; A Shimizu; R F Holcombe; R Eddy; T B Shows; P Leder

The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to Iq21-q23 and RFLP analysis

I Tepler, C C Morton, A Shimizu, R F Holcombe, R Eddy, T B Shows, P Leder

Division of Evolution, Infection and Genomics

Harvard Medical School

Research output: Contribution to journal › Article › peer-review

Abstract

We have used a cDNA probe for the human IgE receptor alpha chain to determine the chromosomal location for the human gene. A combination of Southern blot analysis of panels of somatic-cell hybrid DNAs and chromosomal in situ hybridization has localized the gene to the long arm of chromosome 1 at q21-q23. With this cDNA probe, we have also identified an RsaI RFLP which is inherited in a Mendelian fashion. This polymorphic marker on chromosome 1 should be valuable for studies directed at the identification both of diseases involving the IgE receptor alpha chain and of genetic factors affecting the allergic response.

Original language	English
Pages (from-to)	761-5
Number of pages	5
Journal	American Journal of Human Genetics
Volume	45
Issue number	5
Publication status	Published - Nov 1989

Keywords

Antigens, Differentiation, B-Lymphocyte
Chromosome Mapping
Chromosomes, Human, Pair 1
Cloning, Molecular
DNA Probes
Deoxyribonucleases, Type II Site-Specific
Humans
Hybrid Cells
Immunoglobulin E
Mast Cells
Nucleic Acid Hybridization
Polymorphism, Restriction Fragment Length
Receptors, Fc
Receptors, IgE
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

UN SDGs

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title = "The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to Iq21-q23 and RFLP analysis",

abstract = "We have used a cDNA probe for the human IgE receptor alpha chain to determine the chromosomal location for the human gene. A combination of Southern blot analysis of panels of somatic-cell hybrid DNAs and chromosomal in situ hybridization has localized the gene to the long arm of chromosome 1 at q21-q23. With this cDNA probe, we have also identified an RsaI RFLP which is inherited in a Mendelian fashion. This polymorphic marker on chromosome 1 should be valuable for studies directed at the identification both of diseases involving the IgE receptor alpha chain and of genetic factors affecting the allergic response.",

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author = "I Tepler and Morton, {C C} and A Shimizu and Holcombe, {R F} and R Eddy and Shows, {T B} and P Leder",

year = "1989",

month = nov,

language = "English",

volume = "45",

pages = "761--5",

journal = "American Journal of Human Genetics",

issn = "0002-9297",

publisher = "Cell Press",

number = "5",

}

TY - JOUR

T1 - The gene for the human mast cell high-affinity IgE receptor alpha chain

T2 - chromosomal localization to Iq21-q23 and RFLP analysis

AU - Tepler, I

AU - Morton, C C

AU - Shimizu, A

AU - Holcombe, R F

AU - Eddy, R

AU - Shows, T B

AU - Leder, P

PY - 1989/11

Y1 - 1989/11

N2 - We have used a cDNA probe for the human IgE receptor alpha chain to determine the chromosomal location for the human gene. A combination of Southern blot analysis of panels of somatic-cell hybrid DNAs and chromosomal in situ hybridization has localized the gene to the long arm of chromosome 1 at q21-q23. With this cDNA probe, we have also identified an RsaI RFLP which is inherited in a Mendelian fashion. This polymorphic marker on chromosome 1 should be valuable for studies directed at the identification both of diseases involving the IgE receptor alpha chain and of genetic factors affecting the allergic response.

AB - We have used a cDNA probe for the human IgE receptor alpha chain to determine the chromosomal location for the human gene. A combination of Southern blot analysis of panels of somatic-cell hybrid DNAs and chromosomal in situ hybridization has localized the gene to the long arm of chromosome 1 at q21-q23. With this cDNA probe, we have also identified an RsaI RFLP which is inherited in a Mendelian fashion. This polymorphic marker on chromosome 1 should be valuable for studies directed at the identification both of diseases involving the IgE receptor alpha chain and of genetic factors affecting the allergic response.

KW - Antigens, Differentiation, B-Lymphocyte

KW - Chromosome Mapping

KW - Chromosomes, Human, Pair 1

KW - Cloning, Molecular

KW - DNA Probes

KW - Deoxyribonucleases, Type II Site-Specific

KW - Humans

KW - Hybrid Cells

KW - Immunoglobulin E

KW - Mast Cells

KW - Nucleic Acid Hybridization

KW - Polymorphism, Restriction Fragment Length

KW - Receptors, Fc

KW - Receptors, IgE

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

M3 - Article

C2 - 2573277

SN - 0002-9297

VL - 45

SP - 761

EP - 765

JO - American Journal of Human Genetics

JF - American Journal of Human Genetics

IS - 5

ER -

The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to Iq21-q23 and RFLP analysis

Abstract

Keywords

UN SDGs

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