The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2

Megan Douglas, Chloe O'Loughlin, Adam Lynch, Radvile Prialgauskaite, Antony Adamson, Katharine Dibb, Susan Kimber, Matthew Birket

Research output: Contribution to journalArticlepeer-review

Abstract

The transcription factor NKX2-5 is a highly conserved master regulator of heart
development which is widely expressed in cardiac progenitors and cardiomyocytes. Fluorescent reporters of NKX2-5 that minimally perturb normal protein expression can enable the identification, quantification and isolation of NKX2-5-expressing cells in a normal physiological state. Here we report the generation of two new hESC lines with eGFP inserted upstream (5’) or downstream (3’) of NKX2-5, linked by a cleavable T2A peptide. These complementary reporters produce a robust fluorescent signal in cardiac cells and have wide utility particularly for research on developmental biology and disease modelling.
Original languageEnglish
JournalStem Cell Research
Publication statusAccepted/In press - 21 Nov 2023

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