The Global Characterisation of a Drug-Dendrimer Conjugate - PEGylated poly-lysine Dendrimer

N Akhtar, MB Ashford, L Beer, A Bowes, T Bristow, A Broo, D Buttar, S Coombes, R Cross, E Eriksson, JB Guilbaud, SW Holman, LP Hughes, M Jackman, MJ Lawrence, JSC Lee, WM Li, R Linke, N Mahmoudi, M McCormickB MacMillan, B Newling, M Ngeny, C Patterson, A Poulton, A Ray, N Sanderson, S Sonzini, YY Tang, KE Treacher, D Whittaker, S Wren

Research output: Contribution to journalArticlepeer-review

Abstract

The recent emergence of drug-dendrimer conjugates within pharmaceutical industry research and development introduces a range of challenges for analytical and measurement science. These molecules are very high molecular weight (100-200kDa) with a significant degree of structural complexity. The characteristics and quality attributes that require understanding and definition, and impact efficacy and safety, are diverse. They relate to the intact conjugate, the various building blocks of these complex systems and the level of the free and bound active pharmaceutical ingredient (API). From an analytical and measurement science perspective, this necessitates the measurement of the molecular weight, impurity characterisation, the quantitation of the number of conjugated versus free API molecules, the determination of the impurity profiles of the building blocks, primary structure and both particle size and morphology. Here we report the first example of a global characterisation of a drug-dendrimer conjugate - PEGylated poly-lysine dendrimer currently under development (AZD0466). The impact of the wide variety of analytical and measurement techniques on the overall understanding of this complex molecular entity is discussed, with the relative capabilities of the various approaches compared. The results of this study are an essential platform for the research and development of the future generations of related dendrimer-based medicines.

Original languageEnglish
Pages (from-to)844-858
Number of pages15
JournalJournal of Pharmaceutical Sciences
Volume112
Issue number3
Early online date11 Nov 2022
DOIs
Publication statusPublished - 1 Mar 2023

Keywords

  • Analytical and measurement science
  • Characterisation
  • Drug-dendrimer conjugate

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