The ham-5, rcm-1 and rco-1 genes regulate hyphal fusion in Neurospora crassa

Mash'el Salman Aldabbous, M. Gabriela Roca, Angela Stout, I. Ching Huang, Nick D. Read, Stephen J. Free

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Mutants of Neurospora crassa unable to participate in vegetative hyphal fusion (anastomosis) were isolated and characterized. From this analysis, three genes, rcm-1, rco-1 and ham-5, were identified and shown to be required for hyphal fusion. The rcm-1 and rco-1 genes are homologues of the Saccharomyces cerevisiae SSN6 and TUP1 genes, which encode a dimeric transcription factor in yeast. We demonstrate that in N. crassa the rcm-1 and rco-1 genes are required for hyphal fusion and normal hyphal morphology, and influence both asexual and sexual development. The ham-5 gene encodes a 1686 amino acid protein with two putative WD40 domains, which might participate in protein-protein interactions. ham-5 deletion mutants had a reduced rate of hyphal extension and altered hyphal morphology, and were unable to produce the conidial anastomosis tubes that are required for hyphal fusion during colony initiation. © 2010 SGM.
    Original languageEnglish
    Pages (from-to)2621-2629
    Number of pages8
    JournalMicrobiology
    Volume156
    Issue number9
    DOIs
    Publication statusPublished - Sept 2010

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