The human granulocyte/macrophage colony-stimulating factor receptor α2 isoform influences haemopoietic lineage commitment and divergence

Nicholas J. Slater; Masafumi Yamaguchi; Dominic G. Rothwell; Patrick Baker; Clare M. Heyworth; Rajesh Chopra

doi:10.1046/j.1365-2141.2003.04383.x

The human granulocyte/macrophage colony-stimulating factor receptor α2 isoform influences haemopoietic lineage commitment and divergence

Nicholas J. Slater, Masafumi Yamaguchi, Dominic G. Rothwell, Patrick Baker, Clare M. Heyworth, Rajesh Chopra

Cancer Research UK Manchester Institute

Research output: Contribution to journal › Article › peer-review

Abstract

A number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the α1 and α2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of α1 or α2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-α2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-α1-expressing cells.

Original language	English
Pages (from-to)	150-158
Number of pages	8
Journal	British Journal of Haematology
Volume	122
Issue number	1
DOIs	https://doi.org/10.1046/j.1365-2141.2003.04383.x
Publication status	Published - Jul 2003

Keywords

GM-CSFR
Haemopoietic differentiation
Isoform

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1046/j.1365-2141.2003.04383.x

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title = "The human granulocyte/macrophage colony-stimulating factor receptor α2 isoform influences haemopoietic lineage commitment and divergence",

abstract = "A number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the α1 and α2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of α1 or α2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-α2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-α1-expressing cells.",

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author = "Slater, {Nicholas J.} and Masafumi Yamaguchi and Rothwell, {Dominic G.} and Patrick Baker and Heyworth, {Clare M.} and Rajesh Chopra",

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T1 - The human granulocyte/macrophage colony-stimulating factor receptor α2 isoform influences haemopoietic lineage commitment and divergence

AU - Slater, Nicholas J.

AU - Yamaguchi, Masafumi

AU - Rothwell, Dominic G.

AU - Baker, Patrick

AU - Heyworth, Clare M.

AU - Chopra, Rajesh

PY - 2003/7

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N2 - A number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the α1 and α2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of α1 or α2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-α2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-α1-expressing cells.

AB - A number of alternatively spliced isoforms of haemopoietic growth factor receptors (HGFRs) have been described, but their role in human haemopoiesis remains undetermined. We have investigated the relative expression of the α1 and α2 isoforms of human granulocyte/macrophage colony-stimulating factor receptor (hGM-CSFR) during haemopoietic cell differentiation, and have shown that both subunits are independently regulated during differentiation of CD34+ human haemopoietic progenitor cells. To further investigate these ex-vivo observations, we established a series of murine FDCP mix cell lines, which, as a consequence of the ectopic expression of α1 or α2 hGM-CSFR, demonstrated differential differentiation responses to hGM-CSF. In this model system, hGM-CSFR-α2-expressing cells showed increased hGM-CSF-mediated erythroid/megakaryocytic differentiation compared with hGM-CSFR-α1-expressing cells.

KW - GM-CSFR

KW - Haemopoietic differentiation

KW - Isoform

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DO - 10.1046/j.1365-2141.2003.04383.x

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JO - British Journal of Haematology

JF - British Journal of Haematology

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The human granulocyte/macrophage colony-stimulating factor receptor α2 isoform influences haemopoietic lineage commitment and divergence

Abstract

Keywords

UN SDGs

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