The impact of a human IGF-II analog ([Leu27]IGF-II) on fetal growth in a mouse model of fetal growth restriction

Jayne Charnock; Mark Dilworth; John Aplin; Colin Sibley; Melissa Westwood; Ian P Crocker

doi:10.1152/ajpendo.00379.2015

The impact of a human IGF-II analog ([Leu27]IGF-II) on fetal growth in a mouse model of fetal growth restriction

Jayne Charnock, Mark Dilworth, John Aplin, Colin Sibley, Melissa Westwood, Ian P Crocker

Division of Developmental Biology & Medicine (L5)

Research output: Contribution to journal › Article › peer-review

Abstract

Enhancing placental insulin-like growth factor (IGF) availability appears to be an attractive strategy for improving outcomes in fetal growth restriction (FGR). Our approach was the novel use of [Leu(27)]IGF-II, a human IGF-II analog that binds the IGF-II clearance receptor IGF-IIR in fetal growth-restricted (FGR) mice. We hypothesized that the impact of [Leu(27)]IGF-II infusion in C57BL/6J (wild-type) and endothelial nitric oxide synthase knockout (eNOS(-/-); FGR) mice would be to enhance fetal growth and investigated this from mid- to late gestation; 1 mg·kg(-1)·day(-1) [Leu(27)]IGF-II was delivered via a subcutaneous miniosmotic pump from E12.5 to E18.5. Fetal and placental weights recorded at E18.5 were used to generate frequency distribution curves; fetuses

Original language	English
Pages (from-to)	E24-E31
Journal	American journal of physiology. Endocrinology and metabolism
Volume	310
Issue number	1
DOIs	https://doi.org/10.1152/ajpendo.00379.2015
Publication status	Published - 1 Jan 2016

Keywords

[Leu27]insulin-like growth factor II
endothelial nitric oxide synthase knockout
fetal growth restriction
insulin-like growth factor

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10.1152/ajpendo.00379.2015

Foetal Growth Restriction: A Failure of Placental Adaptation in Response to Foetal Nutrient Demand.
Dilworth, M. (PI)
1/09/13 → 31/08/18
Project: Research

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title = "The impact of a human IGF-II analog ([Leu27]IGF-II) on fetal growth in a mouse model of fetal growth restriction",

abstract = "Enhancing placental insulin-like growth factor (IGF) availability appears to be an attractive strategy for improving outcomes in fetal growth restriction (FGR). Our approach was the novel use of [Leu(27)]IGF-II, a human IGF-II analog that binds the IGF-II clearance receptor IGF-IIR in fetal growth-restricted (FGR) mice. We hypothesized that the impact of [Leu(27)]IGF-II infusion in C57BL/6J (wild-type) and endothelial nitric oxide synthase knockout (eNOS(-/-); FGR) mice would be to enhance fetal growth and investigated this from mid- to late gestation; 1 mg·kg(-1)·day(-1) [Leu(27)]IGF-II was delivered via a subcutaneous miniosmotic pump from E12.5 to E18.5. Fetal and placental weights recorded at E18.5 were used to generate frequency distribution curves; fetuses",

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note = "We would like to acknowledge the support of the Castang Foundation in funding this project, as well as a MRC Programme Grant (Sibley G0802770/92495). MD is supported by a Career Development Fellowship award from the MRC (Grant number MR/K024442/1).",

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AU - Westwood, Melissa

AU - Crocker, Ian P

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The impact of a human IGF-II analog ([Leu27]IGF-II) on fetal growth in a mouse model of fetal growth restriction

Abstract

Keywords

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Foetal Growth Restriction: A Failure of Placental Adaptation in Response to Foetal Nutrient Demand.

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