The influence of lecithin and urea on the in vitro permeation of hydrocortisone acetate through skin from hairless mouse

M. Vitória L B Bentley, Erika R M Kedor, Renata F. Vianna, John H. Collett

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Many workers have attempted to modify the permeability characteristics of skin. In the present work, the in vitro permeation of a model lipophilic drug (hydrocortisone acetate) was studied through hairless mouse skin. The drug was applied in poloxamer 407 gels containing known concentrations of lecithin or urea as penetration enhancers. The results of permeation studies showed that the flux and the retention of the drug were dependent on the concentration of the penetration enhancers. Lecithin (8.0% w/v) caused a retention of seven times more than that of urea (12.0% w/v). The mechanism of penetration enhancement was investigated by differential scanning calorimetry (DSC) and FTIR spectroscopy of the stratum corneum (SC) from hairless mouse treated with solution of lecithin and urea. The lecithin was found to have a significant influence on the lipid matrix of the SC, suggesting a disruption of the intercellular lipid lamellar structure.
    Original languageEnglish
    Pages (from-to)255-262
    Number of pages7
    JournalInternational Journal of Pharmaceutics
    Volume146
    Issue number2
    DOIs
    Publication statusPublished - 15 Jan 1997

    Keywords

    • DSC
    • FTIR
    • Hairless mouse skin
    • Lecithin
    • Penetration enhancers
    • Urea

    Fingerprint

    Dive into the research topics of 'The influence of lecithin and urea on the in vitro permeation of hydrocortisone acetate through skin from hairless mouse'. Together they form a unique fingerprint.

    Cite this