Abstract
This report describes fluorescence decay and time-resolved anisotropy studies of green fluorescent protein (GFP) in various environments. The addition of glucose and fructose, NaCl, or polyethylene glycol changes the viscosity of the medium surrounding the GFP. Both the time-resolved anisotropy and the fluorescence decay of GFP are measured and it is shown that only the time-resolved anisotropy of GFP is affected by the viscosity, but not its fluorescence decay.
| Original language | English |
|---|---|
| Pages (from-to) | 91-95 |
| Number of pages | 4 |
| Journal | Journal of Fluorescence |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 2002 |
Keywords
- Anisotropy
- GFP fluorescence lifetime
- TCSPC
- Viscosity