The local lymph node assay and skin sensitization testing.

Ian Kimber, Rebecca J. Dearman

    Research output: Contribution to journalArticlepeer-review

    Abstract

    The mouse local lymph node assay (LLNA) is a method for the identification and characterization of skin sensitization hazards. In this context the method can be used both to identify contact allergens, and also determine the relative skin sensitizing potency as a basis for derivation of effective risk assessments.The assay is based on measurement of proliferative responses by draining lymph node cells induced following topical exposure of mice to test chemicals. Such responses are known to be causally and quantitatively associated with the acquisition of skin sensitization and therefore provide a relevant marker for characterization of contact allergic potential.The LLNA has been the subject of exhaustive evaluation and validation exercises and has been assigned Organization for Economic Cooperation and Development (OECD) test guideline 429. Herein we describe the conduct and interpretation of the LLNA.
    Original languageEnglish
    Pages (from-to)221-231
    Number of pages10
    JournalMethods in Molecular Biology
    Volume598
    DOIs
    Publication statusPublished - 2010

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