The Location of Iron and Zinc in Grain of Conventional and Biofortified Lines of Sorghum

Anil   Gaddameedi; Sadia Sheraz; Ashok Kumar; Kexue Li; Till   Pellny; Rajeev Gupta; Yongfang Wan; Katie L. Moore; Peter R. Shewry

doi:10.1016/j.jcs.2022.103531

The Location of Iron and Zinc in Grain of Conventional and Biofortified Lines of Sorghum

Anil Gaddameedi, Sadia Sheraz, Ashok Kumar, Kexue Li, Till Pellny, Rajeev Gupta, Yongfang Wan, Katie L. Moore, Peter R. Shewry^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that “hot spots” of ⁵⁶Fe⁺ and ⁶⁴Zn⁺ were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained ³¹P¹⁶O⁺ indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of ⁵⁶Fe⁺ and ⁶⁴Zn⁺ were also observed in the protein matrix of these cells.

Original language	English
Article number	103531
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Cereal Science
Volume	107
Early online date	6 Aug 2022
DOIs	https://doi.org/10.1016/j.jcs.2022.103531
Publication status	Published - 1 Sept 2022

Keywords

Sorghum grain
biofortification
NanoSIMS
localisation
iron
zinc

Research Beacons, Institutes and Platforms

Photon Science Institute

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10.1016/j.jcs.2022.103531Licence: CC BY

Cite this

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title = "The Location of Iron and Zinc in Grain of Conventional and Biofortified Lines of Sorghum",

abstract = "Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that “hot spots” of 56Fe+ and 64Zn+ were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained 31P16O+ indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of 56Fe+ and 64Zn+ were also observed in the protein matrix of these cells.",

keywords = "Sorghum grain, biofortification, NanoSIMS, localisation, iron, zinc",

author = "Anil Gaddameedi and Sadia Sheraz and Ashok Kumar and Kexue Li and Till Pellny and Rajeev Gupta and Yongfang Wan and Moore, \{Katie L.\} and Shewry, \{Peter R.\}",

year = "2022",

month = sep,

day = "1",

doi = "10.1016/j.jcs.2022.103531",

language = "English",

volume = "107",

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journal = "Journal of Cereal Science",

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AU - Gaddameedi, Anil

AU - Sheraz, Sadia

AU - Kumar, Ashok

AU - Li, Kexue

AU - Pellny, Till

AU - Gupta, Rajeev

AU - Wan, Yongfang

AU - Moore, Katie L.

AU - Shewry, Peter R.

PY - 2022/9/1

Y1 - 2022/9/1

N2 - Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that “hot spots” of 56Fe+ and 64Zn+ were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained 31P16O+ indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of 56Fe+ and 64Zn+ were also observed in the protein matrix of these cells.

AB - Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that “hot spots” of 56Fe+ and 64Zn+ were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained 31P16O+ indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of 56Fe+ and 64Zn+ were also observed in the protein matrix of these cells.

KW - Sorghum grain

KW - biofortification

KW - NanoSIMS

KW - localisation

KW - iron

KW - zinc

UR - https://www.scopus.com/pages/publications/85136103937

U2 - 10.1016/j.jcs.2022.103531

DO - 10.1016/j.jcs.2022.103531

M3 - Article

SN - 0733-5210

VL - 107

SP - 1

EP - 7

JO - Journal of Cereal Science

JF - Journal of Cereal Science

M1 - 103531

ER -

The Location of Iron and Zinc in Grain of Conventional and Biofortified Lines of Sorghum

Abstract

Keywords

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Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS)

Surface Characterisation

Cite this

The Location of Iron and Zinc in Grain of Conventional and Biofortified Lines of Sorghum

Abstract

Keywords

Research Beacons, Institutes and Platforms

Access to Document

Other files and links

Embargoed Document

Fingerprint

Equipment

Nanoscale Secondary Ion Mass Spectrometry (NanoSIMS)

Surface Characterisation

Cite this