Abstract

Proteins perform key structural and functional roles in skin. However, there is little consensus as to the identity of the entire skin proteome. For example, estimates of the number of proteins in human skin range from ˜200 for individual mass spectrometry (MS) studies to > 4000 in the protein abundance database (http://pax-db.org/). The lack of consensus may be due to difficulties in extracting dermal extracellular matrix proteins, lack of technique sensitivity and/or specificity, and the use of heterogeneous tissue sources (with regard to donor age, sex and anatomical site), and of cultured skin cells as opposed to skin. In this study we aimed to define the human skin proteome using the most authoritative record of skin composition: the peer-reviewed literature. We have developed a bioinformatics (text mining)-augmented systematic literature review approach to screen original research articles in order to define a healthy human skin proteome. On 31 October 2016 the Web of Science database contained records of > 160 million scientific articles. This database was queried for articles that contained human skin-specific search terms and a reference to at least one human protein (as defined by UniProt: www.uniprot.org). For each protein, articles were manually reviewed to confirm their presence in healthy human skin by appropriate protein identification techniques (MS, immunohistochemistry, western blotting or enzyme-linked immunosorbent assay). For each validated protein we recorded protein name, Uniprot accession number, gene name, skin sample information (age, sex and site), publication ID and tissue localization (if noted). Further information on subcellular location (extracellular matrix, extra- or intracellular) was curated from the Gene Ontology database (www.ebi.ac.uk/QuickGO/). Our resulting validated Manchester Skin Proteome (MSP) contains information on 2950 proteins and is therefore substantially larger than the proteomes defined by single MS studies, or by transcription or immunohistochemistry methods (for example the Human Tissue Atlas lists 441 ‘skin-enriched proteins’). Crucially, 440 proteins in the MSP have not previously been recognized as part of existing skin proteomes. By collating data from peer-reviewed literature our approach to defining tissue proteomes has the potential to overcome both technique- and sample-related limitations inherent in any single study. Well defined tissue and organ proteomes are vital tools in the understanding of biological function, disease pathology and the generation of potential therapy/repair strategies. This work was supported by a Programme Grant awarded by Walgreens Boots Alliance.
Original languageEnglish
PagesE40-E81
DOIs
Publication statusPublished - 18 Apr 2017
EventBritish Society for Investigative Dermatology Annual Meeting 2017 - Hilton Deansgate Hotel, Manchester, United Kingdom
Duration: 3 Apr 20175 Apr 2017

Conference

ConferenceBritish Society for Investigative Dermatology Annual Meeting 2017
Country/TerritoryUnited Kingdom
CityManchester
Period3/04/175/04/17

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