The mTOR-S6 Kinase Pathway Promotes Stress Granule Assembly

Aristeidis Sfakianos, Laura Mellor, Yoke Fei Pang, Paraskevi Kritsiligkou, Hope Needs, Hussein Abou-Hamdan, Laurent Désaubry, Gino Poulin, Mark Ashe, Alan Whitmarsh

Research output: Contribution to journalArticlepeer-review


Stress granules are cytoplasmic mRNA-protein complexes that form upon the inhibition of translation initiation and promote cell survival in response to environmental insults. However, they are often associated with pathologies, including neurodegeneration and cancer, and changes in their dynamics are implicated in ageing. Here we show that the mTOR effector kinases S6 kinase 1 (S6K1) and S6 kinase 2 (S6K2) localise to stress granules in human cells and are required for their assembly and maintenance after mild oxidative stress. The
roles of S6K1 and S6K2 are distinct, with S6K1 having a more significant role in the formation of stress granules via the regulation of eIF2α phosphorylation, while S6K2 is important for their maintenance. In C. elegans, the S6 kinase orthologue RSKS-1 promotes the assembly of stress granules and its loss of function sensitises the nematodes to stress-induced death. This study identifies S6 kinases as regulators of stress granule dynamics and provides a novel link between mTOR signaling, translation inhibition and survival.
Original languageEnglish
Pages (from-to)1766-1780
JournalCell Death and Differentiation
Early online date9 Mar 2018
Publication statusPublished - 2018

Research Beacons, Institutes and Platforms

  • Manchester Institute for Collaborative Research on Ageing


Dive into the research topics of 'The mTOR-S6 Kinase Pathway Promotes Stress Granule Assembly'. Together they form a unique fingerprint.

Cite this