The performance of HE4 alone and in combination with CA125 for the detection of ovarian cancer in an enriched primary care population

Chloe E Barr, Garth Funston, David Jeevan, Sudha Sundar, Luke T. A. Mounce, Emma J. Crosbie

Research output: Contribution to journalArticlepeer-review

Abstract

: Human Epididymis 4 (HE4) is a promising ovarian cancer biomarker, but it has not been evaluated in primary care. In this prospective observational study, we investigated the diagnostic accuracy of HE4 alone and in combination with CA125 for the detection of ovarian cancer in symptomatic women attending primary care. General practitioner (GP)-requested CA125 samples were tested for HE4 at a large teaching hospital in Manchester and cancer outcomes tracked for 12 months. We found a low incidence of ovarian cancer in primary care; thus, the cohort was enriched with pre-surgical samples from 81 ovarian cancer patients. Risk of Ovarian Malignancy Algorithm (ROMA) was calculated using age (</>51) as a surrogate for menopause. Conventional diagnostic accuracy metrics were determined. 1229 patients were included; 82 had ovarian cancer. Overall, ROMA performed best [AUC-0.96 (95%CI: 0.94-0.98, p=<0.001)]. In women under 50-years, the combination of CA125 and HE4 (either marker positive), was superior [sensitivity-100% (95%CI: 81.5-100.0), specificity-80.1% (95%CI 76.7-83.1)]. In women over 50, ROMA performed best [sensitivity-84.4% (95%CI: 73.1-92.2), specificity- 87.2% (95%CI 84.1-90)]. HE4 and ROMA may improve ovarian cancer detection in primary care, particularly for women under 50-years, in whom diagnosis is challenging. Validation in a larger primary care cohort is required.
Original languageEnglish
JournalCancers
Publication statusAccepted/In press - 19 Apr 2022

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