The purification and characterisation of allergenic hazelnut seed proteins

Clare Mills; Neil M. Rigby; Justin Marsh; Ana I. Sancho; Klaus Wellner; Jaap Akkerdaas; Ronald Van Ree; Andre Knulst; Montserrat Fernndez-Rivas; Vlasta Brettlova; Piet P. Schilte; Colin Summer; Richard Pumphrey; Peter R. Shewry; E. N Clare Mills

doi:10.1002/mnfr.200800083

The purification and characterisation of allergenic hazelnut seed proteins

Clare Mills, Neil M. Rigby, Justin Marsh, Ana I. Sancho, Klaus Wellner, Jaap Akkerdaas, Ronald Van Ree, Andre Knulst, Montserrat Fernndez-Rivas, Vlasta Brettlova, Piet P. Schilte, Colin Summer, Richard Pumphrey, Peter R. Shewry, E. N Clare Mills

Research output: Contribution to journal › Article › peer-review

Abstract

A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

Original language	English
Pages (from-to)	S251-S261
Journal	Molecular Nutrition and Food Research
Volume	52
Issue number	2
DOIs	https://doi.org/10.1002/mnfr.200800083
Publication status	Published - Nov 2008

Keywords

Allergens
Characterisation
EuroPrevall
Hazelnut
Purification

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Mills, C., Rigby, N. M., Marsh, J., Sancho, A. I., Wellner, K., Akkerdaas, J., Van Ree, R., Knulst, A., Fernndez-Rivas, M., Brettlova, V., Schilte, P. P., Summer, C., Pumphrey, R., Shewry, P. R., & Mills, E. N. C. (2008). The purification and characterisation of allergenic hazelnut seed proteins. Molecular Nutrition and Food Research, 52(2), S251-S261. https://doi.org/10.1002/mnfr.200800083

@article{3776c03d6aa1437c91a0986b0c10ea33,

title = "The purification and characterisation of allergenic hazelnut seed proteins",

abstract = "A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis. {\textcopyright} 2008 Wiley-VCH Verlag GmbH & Co. KGaA.",

keywords = "Allergens, Characterisation, EuroPrevall, Hazelnut, Purification",

author = "Clare Mills and Rigby, {Neil M.} and Justin Marsh and Sancho, {Ana I.} and Klaus Wellner and Jaap Akkerdaas and {Van Ree}, Ronald and Andre Knulst and Montserrat Fernndez-Rivas and Vlasta Brettlova and Schilte, {Piet P.} and Colin Summer and Richard Pumphrey and Shewry, {Peter R.} and Mills, {E. N Clare}",

note = ", Biotechnology and Biological Sciences Research Council, United Kingdom",

year = "2008",

month = nov,

doi = "10.1002/mnfr.200800083",

language = "English",

volume = "52",

pages = "S251--S261",

journal = "Molecular Nutrition and Food Research",

issn = "1613-4125",

publisher = "John Wiley & Sons Ltd",

number = "2",

}

Mills, C, Rigby, NM, Marsh, J, Sancho, AI, Wellner, K, Akkerdaas, J, Van Ree, R, Knulst, A, Fernndez-Rivas, M, Brettlova, V, Schilte, PP, Summer, C, Pumphrey, R, Shewry, PR & Mills, ENC 2008, 'The purification and characterisation of allergenic hazelnut seed proteins', Molecular Nutrition and Food Research, vol. 52, no. 2, pp. S251-S261. https://doi.org/10.1002/mnfr.200800083

TY - JOUR

T1 - The purification and characterisation of allergenic hazelnut seed proteins

AU - Mills, Clare

AU - Rigby, Neil M.

AU - Marsh, Justin

AU - Sancho, Ana I.

AU - Wellner, Klaus

AU - Akkerdaas, Jaap

AU - Van Ree, Ronald

AU - Knulst, Andre

AU - Fernndez-Rivas, Montserrat

AU - Brettlova, Vlasta

AU - Schilte, Piet P.

AU - Summer, Colin

AU - Pumphrey, Richard

AU - Shewry, Peter R.

AU - Mills, E. N Clare

N1 - , Biotechnology and Biological Sciences Research Council, United Kingdom

PY - 2008/11

Y1 - 2008/11

N2 - A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

AB - A lipid transfer protein (LTP, Cor a 8) together with the 11S (Cor a 9) and 7S seed storage globulins (Cor a 11) are major food allergens present in hazelnut. Methods are described for their purification and characterisation using in-gel tryptic digestion mass spectrometry to confirm their identities and circular dichroism and Fourier-transform infrared spectroscopies to demonstrate that they are authentically folded. Preliminary immunochemical studies have also confirmed that the purified preparations retain their immunological properties in terms of immunoglobulin E binding, determined by immunoblotting using serum from hazelnut allergic patients. These preparations form a basis for development of improved methods of diagnosis of food allergy based on the concept of component-resolved diagnosis. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

KW - Allergens

KW - Characterisation

KW - EuroPrevall

KW - Hazelnut

KW - Purification

U2 - 10.1002/mnfr.200800083

DO - 10.1002/mnfr.200800083

M3 - Article

C2 - 19006093

SN - 1613-4125

VL - 52

SP - S251-S261

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

IS - 2

ER -

The purification and characterisation of allergenic hazelnut seed proteins

Abstract

Keywords

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