The reinnervation and revascularisation pattern of scarless murine fetal wounds

James Henderson, Giorgio Terenghi, Mark William James Ferguson

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    Fetal wounds can heal without scarring. There is evidence that the sensory nervous system plays a role in mediating inflammation and healing, and that the reinnervation pattern of adult wounds differs from that of unwounded skin. Ectoderm is required for development of the cutaneous nerve plexus in early gestation. It was hypothesised that scarless fetal wounds might completely regenerate their neural and vascular architecture. Wounds were made on mouse fetuses at embryonic day16.5 of a 19.5-day gestation, which healed without visible scars. Immunohistochemical analysis of wound sites was performed to assess reinnervation, using antibodies to the pan neuronal marker PGP9.5 as well as to the neuropeptides calcitonin gene-related peptide (CGRP) and substance P (SP). Staining for the endothelial marker von Willebrand factor (VWF) allowed comparison of reinnervation and revascularisation. Wounds were harvested at timepoints from day1 after wounding to postnatal day6. Quantification of wound reinnervation and revascularisation was performed for timepoints up to 6days post-wounding. Hypervascularisation of the wounds occurred within 24h, and blood vessel density within the wounds remained significantly elevated until postnatal day2 (4days post- wounding), after which VWF immunoreactivity was similar between wound and control groups. Wound nerve density returned to a level similar to that of unwounded skin within 48h of wounding, and PGP9.5 immunoreactive nerve fibre density remained similar to control skin thereafter. CGRP and SP immunoreactivity followed a similar pattern to that of PGP9.5, although wound levels did not return to those of control skin until postnatal day1. Scarless fetal wounds appeared to regenerate their nerve and blood vessel microanatomy perfectly after a period of hypervascularisation. © 2011 The Authors. Journal of Anatomy © 2011 Anatomical Society of Great Britain and Ireland.
    Original languageEnglish
    Pages (from-to)660-667
    Number of pages7
    JournalJournal of Anatomy
    Issue number6
    Publication statusPublished - Jun 2011


    • Calcitonin gene-related peptide
    • Fetal wounds
    • Nerves
    • Scarless
    • Substance P
    • Von Willebrand factor


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