TY - JOUR
T1 - The Relationship between Atherosclerosis and Gut Microbiome in Patients with Obstructive Sleep Apnoea
AU - Szabo, Helga
AU - Piroska, Marton
AU - Hernyes, Anita
AU - Zoldi, Luca
AU - Juhasz, Janos
AU - Ligeti, Balazs
AU - Makra, Nora
AU - Szabo, Dora
AU - Bikov, Andras
AU - Kunos, Laszlo
AU - Tarnoki, Adam Domonkos
AU - Tarnoki, David Laszlo
N1 - Funding Information:
This research was funded by the Semmelweis Science and Innovation Fund—Research and Development Application; Hungarian Respiratory Society—Scientific Research Application; Dean’s Fund—Research Application between Theoretical and Clinical Institutes; Bólyai scholarship of the Hungarian Academy of Sciences; ÚNKP-20-5 and ÚNKP-21-5 New National Excellence Program of the Ministry for Innovation and Technology, from the source of the National Research, Development, and Innovation Fund. The bioinformatics analysis was supported by the Hungarian Government grant OTKA 138055 (Large scale surveying of bacteriophages in the human microbiome with pangenomic and machine learning approaches).
Funding Information:
The Samsung ultrasound equipment was provided by Sonarmed Ltd., a Samsung Medison representative. Colleagues of the Medical Imaging Centre and Institute of Medical Microbiology, Semmelweis University, provided administrative and technical support. The authors are grateful to Electro-oxygen for providing equipment for this study and Monika Banlaky for her support with the sleep tests as well as Peter Fussy and Szonja Galyasz for their help during the microbiome measurements, and Bianka Forgo, Marcell Szily and Daniel Tamas Kovacs for their help in the OSA study. Andras Bikov is supported by the NIHR Manchester BRC.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/11/11
Y1 - 2022/11/11
N2 - Background: Obstructive sleep apnoea (OSA) and gut dysbiosis are known risk factors for atherosclerosis. However, only very few studies have been focused on the relationship between OSA, atherosclerosis, and the intestinal microbiome, all in animal models. Methods: Twenty-two patients with OSA, 16 with and 6 without carotid atherosclerosis were involved in the study. After a diagnostic sleep examination, the intima media thickness (IMT) was measured and plaques were found using carotid ultrasound. Blood was also drawn for metabolic profile, and a stool sample was provided for 16S ribosomal RNA microbiome investigation. Results: An increased maximal common carotid artery (CCA) IMT was significantly associated with decreased phylum-level diversity. The level of Peptostreptococcaceae was significantly lower in atherosclerotic subjects. Some other candidate microbes appeared in the two groups at the genus level as well: Bilophila, Romboutsia, Slackia, and Veillonella in the non-atherosclerotic group; and Escherichia-Shigella, Prevotella, and Ruminococcaceae in the atherosclerotic group. Conclusions: This is the first pilot research to analyze the association between the gut microbiome and atherosclerosis in adult patients with OSA with and without carotid atherosclerosis. Dysbiosis and individual bacteria may contribute to the development of carotid atherosclerosis in patients with OSA. Further investigations are necessary to reveal a more precise background in a larger sample.
AB - Background: Obstructive sleep apnoea (OSA) and gut dysbiosis are known risk factors for atherosclerosis. However, only very few studies have been focused on the relationship between OSA, atherosclerosis, and the intestinal microbiome, all in animal models. Methods: Twenty-two patients with OSA, 16 with and 6 without carotid atherosclerosis were involved in the study. After a diagnostic sleep examination, the intima media thickness (IMT) was measured and plaques were found using carotid ultrasound. Blood was also drawn for metabolic profile, and a stool sample was provided for 16S ribosomal RNA microbiome investigation. Results: An increased maximal common carotid artery (CCA) IMT was significantly associated with decreased phylum-level diversity. The level of Peptostreptococcaceae was significantly lower in atherosclerotic subjects. Some other candidate microbes appeared in the two groups at the genus level as well: Bilophila, Romboutsia, Slackia, and Veillonella in the non-atherosclerotic group; and Escherichia-Shigella, Prevotella, and Ruminococcaceae in the atherosclerotic group. Conclusions: This is the first pilot research to analyze the association between the gut microbiome and atherosclerosis in adult patients with OSA with and without carotid atherosclerosis. Dysbiosis and individual bacteria may contribute to the development of carotid atherosclerosis in patients with OSA. Further investigations are necessary to reveal a more precise background in a larger sample.
KW - alpha diversity
KW - carotid atherosclerosis
KW - gut microbiome
KW - intima-media thickness
KW - sleep apnoea
KW - sleep disorder
KW - ultrasound
U2 - 10.3390/app122211484
DO - 10.3390/app122211484
M3 - Article
SN - 2076-3417
VL - 12
JO - Applied Sciences
JF - Applied Sciences
IS - 22
M1 - 11484
ER -