Abstract
We have generated a collection of yeast strains, each of which has an essential yeast gene under the control of the tetracycline-responsive, tetO, promoter. Screens using first-generation promoter-swap strains uncovered the non-specific responsiveness of the tetO7 promoter to a known human transcription factor (hIRF-1). Non-specific regulation was not observed with the tetO2 promoter. Reporter assays have been used to demonstrate this phenomenon. Subsequent efforts to generate a collection of tetracycline-regulatable strains have focused on the tetO2 promoter. These strains are available to the yeast community and can be used for functional genomics studies. Copyright © 2006 John Wiley & Sons, Ltd.
Original language | English |
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Pages (from-to) | 325-331 |
Number of pages | 6 |
Journal | Yeast |
Volume | 23 |
Issue number | 4 |
DOIs | |
Publication status | Published - Mar 2006 |
Keywords
- Essential genes
- Functional genomics
- Saccharomyces cerevisiae
- TetO2
- TetO7
- Tetracycline-regulatable promoter
Research Beacons, Institutes and Platforms
- Manchester Institute of Biotechnology