The relative merits of the tetO2 and tetO7 promoter systems for the functional analysis of heterologous genes in yeast and a compilation of essential yeast genes with tetO2 promoter substitutions

Jill A. Wishart, Michael Osborn, Manda E. Gent, Kuangyu Yen, Zorana Vujovic, Paul Gitsham, Nianshu Zhang, J. Ross Miller, Stephen G. Oliver

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We have generated a collection of yeast strains, each of which has an essential yeast gene under the control of the tetracycline-responsive, tetO, promoter. Screens using first-generation promoter-swap strains uncovered the non-specific responsiveness of the tetO7 promoter to a known human transcription factor (hIRF-1). Non-specific regulation was not observed with the tetO2 promoter. Reporter assays have been used to demonstrate this phenomenon. Subsequent efforts to generate a collection of tetracycline-regulatable strains have focused on the tetO2 promoter. These strains are available to the yeast community and can be used for functional genomics studies. Copyright © 2006 John Wiley & Sons, Ltd.
    Original languageEnglish
    Pages (from-to)325-331
    Number of pages6
    JournalYeast
    Volume23
    Issue number4
    DOIs
    Publication statusPublished - Mar 2006

    Keywords

    • Essential genes
    • Functional genomics
    • Saccharomyces cerevisiae
    • TetO2
    • TetO7
    • Tetracycline-regulatable promoter

    Research Beacons, Institutes and Platforms

    • Manchester Institute of Biotechnology

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