The role of calcium in the volume regulation of rat lacrimal acinar cells

T. Speake, I. J. Douglas, P. D. Brown

    Research output: Contribution to journalArticlepeer-review


    Earlier studies have suggested a role for Ca2+ in regulatory volume decrease (RVD) in response to hypotonic stress through the activation of Ca2+-dependent ion channels (Kotera and Brown, 1993; Park et al., 1994). The involvement of Ca2+ in regulating cell volume in rat lacrimal acinar cells was therefore examined using a video-imaging technique to measure cell volume. The trivalent cation Gd3+ inhibited RVD, suggesting that Ca2+ entry is important and may be via stretch-activated cation channels. However, Fura-2 loaded cells did not show an increase in [Ca2+](i) during exposure to hypotonic solutions, The absence of any changes in [Ca2+](i) resulted from the buffering of cytosolic Ca2+ by Fura-2 during hypotonic shock and therefore inhibition of RVD. The intracellular Ca2+ chelator, BAPTA, also inhibited the RVD response to hypotonic shock. An increase in [Ca2+](i) induced by either acetylcholine or ionomycin, was found to decrease cell volume under isotonic conditions in lacrimal acinar cells. Cell shrinkage was inhibited by tetraethylammonium ion, an inhibitor of Ca2+ activated K+ channels. On the basis of the presented data, we suggest an involvement of intracellular Ca2+ in controlling cell volume in lacrimal acinar cells.
    Original languageEnglish
    Pages (from-to)283-291
    Number of pages8
    JournalJournal of Membrane Biology
    Issue number3
    Publication statusPublished - 1 Aug 1998


    • Acetylcholine
    • Ca2+ signaling
    • Fura-2
    • Regulatory volume decrease
    • Secretion


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